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Western Blot (WB) (Western blot analysis of Calponin-1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human CNN1 Polyclonal Antibody | anti-CNN1 antibody

CNN1, NT (Calponin-1, Calponin H1, Smooth Muscle, Basic Calponin, Calponin 1) (AP)

Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
CNN1; Polyclonal Antibody; NT (Calponin-1; Calponin H1; Smooth Muscle; Basic Calponin; Calponin 1) (AP); anti-CNN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Calponin-1.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-CNN1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-1:50
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human Calponin-1 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Calponin-1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of Calponin-1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-CNN1 antibody
References
1. Marsh, W.L., et.al., Appl. Immunohistochem. Mol. Morphol. 17(3), 216-219 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34.2 kDa (305aa) confirmed by MALDI-TOF
NCBI Official Full Name
calponin-1 isoform 1
NCBI Official Synonym Full Names
calponin 1
NCBI Official Symbol
CNN1
NCBI Official Synonym Symbols
SMCC; Sm-Calp; HEL-S-14
NCBI Protein Information
calponin-1
UniProt Protein Name
Calponin-1
Protein Family
UniProt Gene Name
CNN1
UniProt Entry Name
CNN1_HUMAN

Uniprot Description

calponin 1: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.

Protein type: Actin-binding; Contractile; Myosin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: focal adhesion; cytoskeleton

Molecular Function: calmodulin binding; actin binding

Biological Process: regulation of smooth muscle contraction; actomyosin structure organization and biogenesis

Research Articles on CNN1

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Product Notes

The CNN1 cnn1 (Catalog #AAA6466844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CNN1, NT (Calponin-1, Calponin H1, Smooth Muscle, Basic Calponin, Calponin 1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CNN1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-1:50 ELISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CNN1 cnn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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