anti-Rat CNG4 Polyclonal Antibody | anti-CNG4 antibody

Anti - CNG4 (Cyclic Nucleotide-Gated Channels 4, b-Subunit)

Cat. Num. AAA614299

• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Serum
• Synonyms: CNG4; Polyclonal Antibody; Anti - CNG4 (Cyclic Nucleotide-Gated Channels 4; b-Subunit); CNG4 (Cyclic Nucleotide-Gated Channels 4; anti-CNG4 antibody

• Host: Host: Rabbit; Source: Rat
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The 24 AA rat control peptide is 100% conserved in alternatively spliced rat b-subunits (1339 aa & 858 aa) and 66% in human b-subunits (1251 aa, RCNC2a 623 aa, 1245 b1 and RCNC2b 909 aa). No significant sequence homology is detected with other CNGs. The control peptide, because of small size (MW 2.65kD), is not suitable for Western. It should be used for ELISA and antibody blocking to confirm specificity of antibodies
• Purity/Purification: Serum
• Form/Format: Serum; Supplied as a liquid, neat serum, 40% glycerol.

• Applicable Applications for anti-CNG4 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.; ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.; Optimal dilutions to be determined by the researcher.
• Immunogen: A 24 AA Peptide sequence near C-terminus of rat CNG4 (1) was used for antibody production. The peptide was coupled to KLH
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CNG4 antibody

The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated a and b subunits. Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves, whereas, b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits.

Product Categories/Family for anti-CNG4 antibody

Abs to Ion Channel

References

• (1). Sautter A et al (1997) Mol. Brain Res. 47, 171; Sautter A et al (1998) PNAS 95, 4696; Chen TY et al (1993) Nature 362, 764, Ardell MD et al (1996) FEBS Lett. 389, 213; Koerschen HG et al (1995) Neuron 15, 627; Biel M et al (1996) J Biol. Chem. 271, 6349; Bognik W et al (1999) J Neurosci. 19, 5332; Colville CA et al (1996) J Biol. Chem. 271, 32968