Rabbit anti-Human CMKLR1 Polyclonal Antibody | anti-CMKLR1 antibody

CMKLR1, CT (CMKLR1, CHEMR23, DEZ, Chemokine-like receptor 1, G-protein coupled receptor ChemR23, G-protein coupled receptor DEZ) (FITC)

Cat. Num. AAA6287034

• Gene Names: CMKLR1; DEZ; RVER1; ChemR23; CHEMERINR
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CMKLR1; Polyclonal Antibody; CT (CMKLR1; CHEMR23; DEZ; Chemokine-like receptor 1; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ) (FITC); G-protein coupled receptor DEZ; anti-CMKLR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CMKLR1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CMKLR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-333 amino acids from the C-terminal region of human CMKLR1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CMKLR1 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CMKLR1 antibody detected the CMKLR1 protein (arrow).)

Related Product Information for anti-CMKLR1 antibody

Orphan receptor. Could be a chemotactic peptide receptor. May have a function in bone metabolism. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).

Product Categories/Family for anti-CMKLR1 antibody

Antibodies; G Protein Receptors

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Davila, S., et al. Genes Immun. 11(3):232-238(2010). Ohira, T., et al. J. Biol. Chem. 285(5):3451-3461(2010). Kaur, J., et al. Biochem. Biophys. Res. Commun. 391(4):1762-1768(2010). Skrzeczynska-Moncznik, J., et al. Biochem. Biophys. Res. Commun. 380(2):323-327(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4758014
• NCBI GeneID: 1240
• NCBI Accession #: NP_004063.1
• NCBI GenBank Nucleotide #: NM_004072.2
• UniProt Accession #: Q99788; O75748; Q3KP37; Q5U0H0; Q99789; A8K6Y5
• Molecular Weight: 42,035 Da
• NCBI Official Full Name: chemokine-like receptor 1 isoform b
• NCBI Official Synonym Full Names: chemokine-like receptor 1
• NCBI Official Symbol: CMKLR1
• NCBI Official Synonym Symbols: DEZ; RVER1; ChemR23; CHEMERINR
• NCBI Protein Information: chemokine-like receptor 1; chemerin receptor; resolvin E1 receptor; chemokine receptor-like 1; G-protein coupled receptor DEZ; G-protein coupled receptor ChemR23; orphan G-protein coupled receptor, Dez
• UniProt Protein Name: Chemokine-like receptor 1
• UniProt Gene Name: CMKLR1
• UniProt Synonym Gene Names: CHEMR23; DEZ
• UniProt Entry Name: CML1_HUMAN

Uniprot Description

CMKLR1: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF- kappa-B. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2). Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; chemokine receptor activity; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of interleukin-12 production; inhibition of NF-kappaB transcription factor; regulation of calcium-mediated signaling; positive regulation of fat cell differentiation; immune response; chemotaxis; skeletal development