Western Blot (WB)
(Western Blot: Sample: Human Serum Tissue.)
Rabbit anti-Human Clusterin (CLU) Polyclonal Antibody | anti-CLU antibody
Polyclonal Antibody to Clusterin (CLU)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Human Serum Tissue.)
Western Blot (WB)
(Western Blot: Sample: Human Serum Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CLU, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CLU, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]
Uniprot Description
CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Mitochondrial; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p21.1
Cellular Component: cell surface; cytoplasm; cytosol; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; intracellular; mitochondrial membrane; mitochondrion; nucleus; perinuclear region of cytoplasm; protein complex
Molecular Function: amyloid-beta binding; ATPase activity; chaperone binding; low-density lipoprotein receptor binding; misfolded protein binding; protein binding; protein complex binding; ubiquitin protein ligase binding
Biological Process: antimicrobial humoral response; cell morphogenesis; central nervous system myelin maintenance; chaperone-mediated protein complex assembly; complement activation; complement activation, classical pathway; innate immune response; lipid metabolic process; microglial cell activation; negative regulation of protein homooligomerization; platelet degranulation; positive regulation of NF-kappaB transcription factor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein homooligomerization; positive regulation of receptor-mediated endocytosis; positive regulation of tumor necrosis factor production; protein import; protein stabilization; regulation of complement activation; release of cytochrome c from mitochondria; response to misfolded protein; response to virus; reverse cholesterol transport
Research Articles on CLU
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Product Notes
The CLU clu (Catalog #AAA2003051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Clusterin (CLU) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Clusterin (CLU) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP), ELISA. Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CLU clu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Clusterin (CLU), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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