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Western Blot (WB) (Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Lung Tissue.)

Rabbit anti-Human Cluster Of Differentiation 200 Polyclonal Antibody | anti-CD200 antibody

Polyclonal Antibody to Cluster Of Differentiation 200 (CD200)

Gene Names
CD200; MRC; MOX1; MOX2; OX-2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Cluster Of Differentiation 200; Polyclonal Antibody; Polyclonal Antibody to Cluster Of Differentiation 200 (CD200); anti-CD200 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CD200. It has beenselected for its ability to recognize CD200 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.21mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QVQVVTQDER EQLYTPASLK CSLQNAQEAL IVTWQKKKAV SPENMVTFSE NHGVVIQPAY KDKINITQLG LQNSTITFWN ITLEDEGCYM CLFNTFGFGK ISGTACLTVY VQPIVSLHYK FSEDHLNITC SATARPAPMV FWKVPRSGIE NSTVTLSHPN GTTSVTSILH IKDPKNQVGK EVICQVLHLG TVTDFKQTVN
Sequence Length
269
Applicable Applications for anti-CD200 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
CD200 (Gln31~Gly232)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant CD200 (Gln31~Gly232) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2049058
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Lung Tissue.)

Western Blot (WB) (Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Lung Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant CD200, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant CD200, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,975 Da
NCBI Official Full Name
OX-2 membrane glycoprotein isoform b
NCBI Official Synonym Full Names
CD200 molecule
NCBI Official Symbol
CD200
NCBI Official Synonym Symbols
MRC; MOX1; MOX2; OX-2
NCBI Protein Information
OX-2 membrane glycoprotein
UniProt Protein Name
OX-2 membrane glycoprotein
UniProt Gene Name
CD200
UniProt Synonym Gene Names
MOX1; MOX2

NCBI Description

This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

CD200: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cell-cell adherens junction; integral component of plasma membrane; plasma membrane

Molecular Function: cell adhesion molecule binding; protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: cell recognition; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; homophilic cell adhesion; negative regulation of macrophage activation; regulation of immune response

Research Articles on CD200

Similar Products

Product Notes

The CD200 cd200 (Catalog #AAA2027034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cluster Of Differentiation 200 (CD200) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cluster Of Differentiation 200 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CD200 cd200 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QVQVVTQDER EQLYTPASLK CSLQNAQEAL IVTWQKKKAV SPENMVTFSE NHGVVIQPAY KDKINITQLG LQNSTITFWN ITLEDEGCYM CLFNTFGFGK ISGTACLTVY VQPIVSLHYK FSEDHLNITC SATARPAPMV FWKVPRSGIE NSTVTLSHPN GTTSVTSILH IKDPKNQVGK EVICQVLHLG TVTDFKQTVN. It is sometimes possible for the material contained within the vial of "Cluster Of Differentiation 200, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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