Western Blot (WB)
(Western blot analysis of CLTB (arrow) using rabbit polyclonal CLTB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTB gene.)
Rabbit anti-Human CLTB Polyclonal Antibody | anti-CLTB antibody
CLTB (CLTB, Clathrin light chain B) (FITC)
Gene Names
CLTB; LCB
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CLTB; Polyclonal Antibody; CLTB (CLTB; Clathrin light chain B) (FITC); Clathrin light chain B; anti-CLTB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-CLTB antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
CLTB antibody is generated from rabbits immunized with a recombinant protein from human CLTB.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CLTB (arrow) using rabbit polyclonal CLTB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTB gene.)
Western Blot (WB)
(Western blot analysis of CLTB (arrow) using rabbit polyclonal CLTB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTB gene.)
Related Product Information for anti-CLTB antibody
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-CLTB antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Martins-de-Souza, D., et al. J Neural Transm 116(3):275-289(2009). Loerke, D., et al. Traffic 6(10):918-929(2005). Chen, C.Y., et al. J. Biol. Chem. 280(7):6109-6117(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,190 Da
NCBI Official Full Name
clathrin light chain B isoform b
NCBI Official Synonym Full Names
clathrin, light chain B
NCBI Official Symbol
CLTB
NCBI Official Synonym Symbols
LCB
NCBI Protein Information
clathrin light chain B; OTTHUMP00000161245; OTTHUMP00000161246; clathrin, light chain (Lcb); clathrin, light polypeptide (Lcb)
UniProt Protein Name
Clathrin light chain B
Protein Family
UniProt Gene Name
CLTB
UniProt Synonym Gene Names
Lcb
UniProt Entry Name
CLCB_HUMAN
Uniprot Description
CLTB: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: clathrin coat of trans-Golgi network vesicle; clathrin coat; intracellular membrane-bound organelle; cytoplasm; plasma membrane; trans-Golgi network; clathrin coat of coated pit
Molecular Function: protein binding; structural molecule activity; peptide binding
Biological Process: vesicle-mediated transport; intracellular protein transport
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Product Notes
The CLTB cltb (Catalog #AAA6286968) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLTB (CLTB, Clathrin light chain B) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLTB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CLTB cltb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLTB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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