Rabbit CLK2 Polyclonal Antibody | anti-CLK2 antibody

CLK2 Antibody

Cat. Num. AAA9505082

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: CLK2; Polyclonal Antibody; CLK2 Antibody; CDC-like kinase 2; kinase CLK2; anti-CLK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: CLK2 Antibody detects endogenous levels of total CLK2 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 498

• Applicable Applications for anti-CLK2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human CLK2.
• Immunogen Range: Jan-50
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from COLO205 and A549 cells, using CLK2 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 661567373
• NCBI GeneID: 1196
• NCBI Accession #: NP_001281267.1
• NCBI GenBank Nucleotide #: NM_001294338.1
• UniProt Accession #: P49760; Q96CQ0; B1AVS9; B5MBX6
• Molecular Weight: 60 kDa
• NCBI Official Full Name: dual specificity protein kinase CLK2 isoform 1
• NCBI Official Synonym Full Names: CDC like kinase 2
• NCBI Official Symbol: CLK2
• NCBI Protein Information: dual specificity protein kinase CLK2
• UniProt Protein Name: Dual specificity protein kinase CLK2
• Protein Family: Dual specificity protein kinase
• UniProt Gene Name: CLK2

NCBI Description

This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.