Rabbit CLIC1 Polyclonal Antibody | anti-CLIC1 antibody

CLIC1 Antibody

Cat. Num. AAA9629274

• Gene Names: CLIC1; G6; NCC27
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CLIC1; Polyclonal Antibody; CLIC1 Antibody; Chloride channel ABP; Chloride intracellular channel 1; Chloride intracellular channel protein 1; CLIC 1; CLIC1_HUMAN; G6; hRNCC; NCC 27; NCC27; Nuclear chloride ion channel 27; Nuclear chloride ion channel protein; p64CLCP; Regulatory nuclear chloride ion channel protein; RNCC protein; anti-CLIC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CLIC1 Antibody detects endogenous levels of total CLIC1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CLIC1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion. Belongs to the chloride channel CLIC family.
• Post Translational Modifications: Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
• Subunit Structure: Monomer. Homodimer (in vitro). Interacts with TRAPPC2. Dimerization requires a conformation change that leads to the exposure of a large hydrophobic surface. In vivo, this may lead to membrane insertion. Interacts with AKAP9.
• Tissue Specificity: Expression is prominent in heart, placenta, liver, kidney and pancreas.
• Function: Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
• Note: Mostly in the nucleus including in the nuclear membrane (PubMed:9139710, PubMed:12681486). Small amount in the cytoplasm and the plasma membrane (PubMed:9139710). Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (PubMed:11940526, PubMed:11551966, PubMed:14613939).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CLIC1 antibody

Nucleus; Nucleus membrane; Single-pass membrane protein; Cytoplasm; Cell membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 567757607
• NCBI GeneID: 1192
• NCBI Accession #: NP_001274522.1
• NCBI GenBank Nucleotide #: NM_001287593.1
• UniProt Accession #: O00299; Q15089; Q502X1
• Molecular Weight: 26,923 Da
• NCBI Official Full Name: chloride intracellular channel protein 1
• NCBI Official Synonym Full Names: chloride intracellular channel 1
• NCBI Official Symbol: CLIC1
• NCBI Official Synonym Symbols: G6; NCC27
• NCBI Protein Information: chloride intracellular channel protein 1; RNCC protein; chloride channel ABP; hRNCC; nuclear chloride ion channel 27; nuclear chloride ion channel protein; p64CLCP; regulatory nuclear chloride ion channel protein
• UniProt Protein Name: Chloride intracellular channel protein 1
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC1
• UniProt Synonym Gene Names: G6; NCC27; NCC27; hRNCC
• UniProt Entry Name: CLIC1_HUMAN

NCBI Description

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008]

Uniprot Description

CLIC1: Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle. Belongs to the chloride channel CLIC family.

Protein type: Membrane protein, integral; Channel, chloride

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; nuclear membrane; mitochondrion; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nuclear envelope; nucleus; vesicle; brush border

Molecular Function: chloride channel activity; protein binding; voltage-gated ion channel activity

Biological Process: positive regulation of osteoblast differentiation; regulation of mitochondrial membrane potential; regulation of cell cycle; chloride transport; signal transduction