Western Blot (WB)
(Western blot analysis of CLEC3B (arrow) using rabbit polyclonal CLEC3B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLEC3B gene.)
Rabbit anti-Human CLEC3B Polyclonal Antibody | anti-CLEC3B antibody
CLEC3B, ID (CLEC3B, TNA, Tetranectin, C-type lectin domain family 3 member B, Plasminogen kringle 4-binding protein) (PE)
Gene Names
CLEC3B; TN; TNA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
CLEC3B; Polyclonal Antibody; ID (CLEC3B; TNA; Tetranectin; C-type lectin domain family 3 member B; Plasminogen kringle 4-binding protein) (PE); Plasminogen kringle 4-binding protein; anti-CLEC3B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CLEC3B antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:50-100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
CLEC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human CLEC3B.
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CLEC3B (arrow) using rabbit polyclonal CLEC3B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLEC3B gene.)
Western Blot (WB)
(Western blot analysis of CLEC3B (arrow) using rabbit polyclonal CLEC3B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLEC3B gene.)
Immunohistochemistry (IHC)
(CLEC3B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CLEC3B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CLEC3B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CLEC3B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CLEC3B Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CLEC3B Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CLEC3B antibody
Tetranectin binds to plasminogen and to isolated kringle 4. It may be involved in the packaging of molecules destined for exocytosis.
Product Categories/Family for anti-CLEC3B antibody
References
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Valdes, A.M., et al. Arthritis Rheum. 54(2):533-539(2006). Hermann, M., et al. Transplant. Proc. 37(2):1322-1325(2005). Anderson, N.L., et al. Mol. Cell Proteomics 3(4):311-326(2004). Westergaard, U.B., et al. Eur. J. Biochem. 270(8):1850-1854(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,537 Da
NCBI Official Full Name
tetranectin isoform 1precursor
NCBI Official Synonym Full Names
C-type lectin domain family 3 member B
NCBI Official Symbol
CLEC3B
NCBI Official Synonym Symbols
TN; TNA
NCBI Protein Information
tetranectin
UniProt Protein Name
Tetranectin
Protein Family
UniProt Gene Name
CLEC3B
UniProt Synonym Gene Names
TNA; TN
UniProt Entry Name
TETN_HUMAN
Uniprot Description
CLEC3B: Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p22-p21.3
Cellular Component: extracellular matrix; extracellular space; granular component; cytoplasm; extracellular region
Molecular Function: heparin binding; calcium ion binding; carbohydrate binding
Biological Process: ossification; skeletal development; bone mineralization
Research Articles on CLEC3B
Similar Products
Product Notes
The CLEC3B clec3b (Catalog #AAA6286689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLEC3B, ID (CLEC3B, TNA, Tetranectin, C-type lectin domain family 3 member B, Plasminogen kringle 4-binding protein) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC3B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:10-50 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CLEC3B clec3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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