Rabbit Cleaved-Caspase 7 Polyclonal Antibody | anti-CASP7 antibody

Cleaved-Caspase 7 (Asp198) Antibody

Cat. Num. AAA9613061

• Gene Names: CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• Reactivity: Human, Mouse; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (86%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: Cleaved-Caspase 7; Polyclonal Antibody; Cleaved-Caspase 7 (Asp198) Antibody; Apoptotic protease Mch-3; Apoptotic protease MCH3; CASP-7; CASP7; CASP7_HUMAN; Caspase 7; Caspase 7 apoptosis related cysteine peptidase; Caspase-7 subunit p11; Caspase7; CMH 1; CMH-1; CMH1; ICE LAP3; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3; anti-CASP7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (86%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Cleaved-Caspase 7 (Asp198) Antibody detects endogenous levels of fragment of activated Caspase 7 resulting from cleavage adjacent to Asp198.; Tissue Specificity: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CASP7 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Caspase 7 (Cleaved-Asp198).
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
• Subunit Structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20kDa (p20) and a 11kDa (p11) subunit. Interacts with BIRC6/bruce. Interacts with ATXN3 (short isoform 1).
• Similarity: Belongs to the peptidase C14A family.
• Subcellular Location: Cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from MCF7, using Cleaved Caspase-7 (Asp198). The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)

Related Product Information for anti-CASP7 antibody

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 4502581
• NCBI GeneID: 840
• NCBI Accession #: NP_001218.1
• NCBI GenBank Nucleotide #: NM_001227.4
• UniProt Accession #: P55210; Q13364; Q53YD5; Q5SVL0; Q5SVL3; Q96BA0; B4DQU7; B5BU45; D3DRB8
• Molecular Weight: 34,277 Da
• NCBI Official Full Name: caspase-7 isoform alpha
• NCBI Official Synonym Full Names: caspase 7, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP7
• NCBI Official Synonym Symbols: MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• NCBI Protein Information: caspase-7; apoptotic protease MCH-3; ICE-like apoptotic protease 3; caspase 7, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-7
• Protein Family: Caspase
• UniProt Gene Name: CASP7
• UniProt Synonym Gene Names: MCH3; CASP-7; ICE-LAP3
• UniProt Entry Name: CASP7_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

CASP7: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly- 217' bond. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.60; Endoplasmic reticulum; Protease; Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: apoptosis; caspase activation via cytochrome c; proteolysis; cell structure disassembly during apoptosis