Rabbit anti-Human CLDN7 Polyclonal Antibody | anti-CLDN7 antibody

CLDN7, CT (CLDN7, CEPTRL2, CPETRL2, Claudin-7) (Biotin)

Cat. Num. AAA6286527

• Gene Names: CLDN7; CLDN-7; CEPTRL2; CPETRL2; Hs.84359; claudin-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CLDN7; Polyclonal Antibody; CT (CLDN7; CEPTRL2; CPETRL2; Claudin-7) (Biotin); Claudin-7; anti-CLDN7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLDN7.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CLDN7 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 172-201 in the C-terminal region of human CLDN7.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS6002466. This demonstrates that MBS6002466 detected the CLDN7 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma using MBS6002466 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6002466 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using MBS6002466 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CLDN7 antibody

Antibodies; Claudin

References

1. Kojima, F., et al. Oncol. Rep. 23(4):927-931(2010). 2. Rendon-Huerta, E., et al. J Gastrointest Cancer 41(1):52-59(2010). 3. Ouban, A., et al. Histol. Histopathol. 25(1):83-90(2010). 4. Kaarteenaho, R., et al. Respir. Res. 11, 59 (2010). 5. Lal-Nag, M., et al. Genome Biol. 10 (8), 235 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 297206811
• NCBI GeneID: 1366
• NCBI Accession #: NP_001171951.1; NP_001298.3
• NCBI GenBank Nucleotide #: NM_001185022.1
• UniProt Accession #: Q95471; Q6IPN3; Q7Z4Y7; Q9BVN0; B2R9X7; D3DTP0; O95471
• Molecular Weight: 22,418 Da
• NCBI Official Full Name: claudin-7 isoform 1
• NCBI Official Synonym Full Names: claudin 7
• NCBI Official Symbol: CLDN7
• NCBI Official Synonym Symbols: CLDN-7; CEPTRL2; CPETRL2; Hs.84359; claudin-1
• NCBI Protein Information: claudin-7; clostridium perfringens enterotoxin receptor-like 2
• Protein Family: Claudin

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc.. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]