Rabbit anti-Human CLDN2 Polyclonal Antibody | anti-CLDN2 antibody

CLDN2, CT (Y224) (CLDN2, Claudin-2, SP82) (FITC)

Cat. Num. AAA6286473

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CLDN2; Polyclonal Antibody; CT (Y224) (CLDN2; Claudin-2; SP82) (FITC); SP82; anti-CLDN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLDN2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CLDN2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 200-227 in the C-terminal region of human CLDN2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of human heart lysate 20ug per lane usingMBS6010363. Secondary IgG (H+L) (HRP) goat anti-rabbit Predicted band size: 25kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis of CLDN2 (arrow) usingMBS6010363. Lane 1: 293 cell lysates (2ug/lane) Lane 2: transiently transfected with the CLDN2 gene.)

Immunohistochemistry (IHC)

(immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS6010363 followed by peroxidase conjugation of the secondary antibody and DAB staining..)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of 293 cells (right histogram) using MBS6010363 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CLDN2 antibody

Antibodies; Claudin

References

1. Smith, A.J., et al. J. Acquir. Immune Defic. Syndr. 55(3):306-315(2010). 2. Kojima, F., et al. Oncol. Rep. 23(4):927-931(2010). 3. Szakal, D.N., et al. Virchows Arch. 456(3):245-250(2010). 4. Buchert, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(6):2628-2633(2010). 5. Mankertz, J., et al. Cell Tissue Res. 336(1):67-77(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 283806634
• NCBI GeneID: 9075
• NCBI Accession #: NP_001164566.1; NP_001164563.1
• NCBI GenBank Nucleotide #: NM_001171095.1
• UniProt Accession #: P57739; B2R6B9
• Molecular Weight: 24,549 Da
• NCBI Official Full Name: claudin-2
• NCBI Official Synonym Full Names: claudin 2
• NCBI Official Symbol: CLDN2
• NCBI Protein Information: claudin-2
• UniProt Protein Name: Claudin-2
• Protein Family: Claudin
• UniProt Gene Name: CLDN2
• UniProt Entry Name: CLD2_HUMAN

NCBI Description

This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

Claudin-2: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cytoskeletal

Chromosomal Location of Human Ortholog: Xq22.3-q23

Cellular Component: tight junction; plasma membrane; integral to membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion