Rabbit anti-Human CLDN2 Polyclonal Antibody | anti-CLDN2 antibody

CLDN2 Antibody (C-term Y224)

Cat. Num. AAA9203312

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CLDN2; Polyclonal Antibody; CLDN2 Antibody (C-term Y224); Claudin-2; SP82; anti-CLDN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This CLDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the C-terminal region of human CLDN2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 230

• Applicable Applications for anti-CLDN2 antibody: WB, IHC-P, FC, E
• Application Notes: WB~~1:1000; IHC-P~~1:10~50; FC~~1:10~50
• Calculated MW: 24549
• Antigen Region: 200-227
• Function: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity.
• Cellular Location: Cell junction, tight junction. Cell membrane;Multi-pass membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Anti-CLDN2 A

(Anti-CLDN2 Anti-CLDN2 Antibody at 1:1000 dilution +human heart lysate Lysates/proteins at 20 µgper lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of CLDN2 (arrow) using rabbit polyclonal CLDN2 Antibody (Y224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.)

Immunohistochemistry (IHC)

(CLDN2 Antibody (C-term Y224) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CLDN2 Antibody (C-term Y224) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CLDN2 Antibody (C-term Y224) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CLDN2 antibody

This gene product belongs to the claudin protein familywhose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.

Product Categories/Family for anti-CLDN2 antibody

Signal Transduction

References

Smith, A.J., et al. J. Acquir. Immune Defic. Syndr. 55(3):306-315(2010)
Kojima, F., et al. Oncol. Rep. 23(4):927-931(2010)
Szakal, D.N., et al. Virchows Arch. 456(3):245-250(2010)
Buchert, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(6):2628-2633(2010)
Mankertz, J., et al. Cell Tissue Res. 336(1):67-77(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 283806628
• NCBI GeneID: 9075
• NCBI Accession #: NP_001164563.1; NP_001164566.1; NP_065117.1
• NCBI GenBank Nucleotide #: NM_001171092.1
• UniProt Accession #: P57739; B2R6B9
• NCBI Official Full Name: claudin-2
• NCBI Official Synonym Full Names: claudin 2
• NCBI Official Symbol: CLDN2
• NCBI Protein Information: claudin-2
• UniProt Protein Name: Claudin-2
• Protein Family: Claudin
• UniProt Gene Name: CLDN2
• UniProt Entry Name: CLD2_HUMAN

NCBI Description

This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

Claudin-2: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.

Protein type: Membrane protein, multi-pass; Cytoskeletal; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq22.3-q23

Cellular Component: tight junction; integral to membrane; plasma membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion