Rabbit anti-Human, Mouse CLCN2 Polyclonal Antibody | anti-CLCN2 antibody

CLCN2, NT (CLCN2, Chloride channel protein 2) (FITC)

Cat. Num. AAA6286352

• Gene Names: CLCN2; CLC2; ECA2; ECA3; EGI3; EGMA; EJM6; EJM8; CIC-2; EGI11; HALD2; LKPAT; clC-2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLCN2; Polyclonal Antibody; NT (CLCN2; Chloride channel protein 2) (FITC); Chloride channel protein 2; anti-CLCN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 854

• Applicable Applications for anti-CLCN2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CLCN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-237 amino acids from the N-terminal region of human CLCN2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CLCN2 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CLCN2 antibody detected the CLCN2 protein (arrow).)

Western Blot (WB)

(CLCN2 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the CLCN2 antibody detected the CLCN2 protein (arrow).)

Related Product Information for anti-CLCN2 antibody

The transmembrane protein encoded by this gene is a voltage-gated chloride channel that maintains chloride ion homeostasis in various cells. Defects in this gene may be a cause of certain epilepsies. Four transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CLCN2 antibody

Antibodies; Ion Channel

References

Scheper, G.C., et al. Genet Test Mol Biomarkers 14(2):255-257(2010). Cornejo, I., et al. J. Cell. Physiol. 221(3):650-657(2009). Thompson, C.H., et al. J. Biol. Chem. 284(38):26051-26062(2009). Kleefuss-Lie, A., et al. Nat. Genet. 41(9):954-955(2009). Klaus, F., et al. Biochem. Biophys. Res. Commun. 381(3):407-411(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 283806618
• NCBI GeneID: 1181
• NCBI Accession #: NP_001164559.1
• NCBI GenBank Nucleotide #: NP_001164559.1
• UniProt Accession #: P51788
• NCBI Official Full Name: chloride channel protein 2 isoform 3
• NCBI Official Synonym Full Names: chloride voltage-gated channel 2
• NCBI Official Symbol: CLCN2
• NCBI Official Synonym Symbols: CLC2; ECA2; ECA3; EGI3; EGMA; EJM6; EJM8; CIC-2; EGI11; HALD2; LKPAT; clC-2
• NCBI Protein Information: chloride channel protein 2
• UniProt Protein Name: Chloride channel protein 2
• Protein Family: Chloride channel protein
• UniProt Gene Name: CLCN2
• UniProt Synonym Gene Names: ClC-2
• UniProt Entry Name: CLCN2_HUMAN

NCBI Description

This gene encodes a voltage-gated chloride channel. The encoded protein is a transmembrane protein that maintains chloride ion homeostasis in various cells. Defects in this gene may be a cause of certain epilepsies. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

CLCN2: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. Defects in CLCN2 are associated with susceptibility to epilepsy, idiopathic generalized type 11 (EIG11). A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. Defects in CLCN2 are associated with juvenile absence epilepsy type 2 (JAE2). JAE is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset occurring around puberty, absence seizures, generalized tonic- clonic seizures (GTCS), GTCS on awakening and myoclonic seizures. Defects in CLCN2 are associated with juvenile myoclonic epilepsy type 8 (EJM8). A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. Belongs to the chloride channel (TC 2.A.49) family. ClC-2/CLCN2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, chloride; Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: plasma membrane

Molecular Function: voltage-gated chloride channel activity

Biological Process: retina development in camera-type eye; transport; transmembrane transport

Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 11; Leukoencephalopathy With Ataxia