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Western Blot (WB) (Western blot analysis of extracts from HepG2 and rat heart, using CLCC1 Antibody.)

Rabbit anti-Human, Rat CLCC1 Polyclonal Antibody | anti-CLCC1 antibody

CLCC1 Antibody

Gene Names
CLCC1; MCLC
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CLCC1; Polyclonal Antibody; CLCC1 Antibody; Chloride channel CLIC like protein 1; KIAA0761; MCLC; Mid 1 related chloride channel 1; Mid 1 related chloride channel protein 1; OTTMUSP00000007700; OTTMUSP00000007701; RP23-217I18.1; anti-CLCC1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CLCC1 antibody detects endogenous levels of total CLCC1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
551
Applicable Applications for anti-CLCC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Membrane; Multipass Membrane Protein Potential. Endoplasmic Reticulum. Golgi Apparatus. Nucleus.
Predicted Cross Reactivity
Bovine, Horse, Sheep
Similarity
Bovine (82%), Horse (82%), Sheep (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 and rat heart, using CLCC1 Antibody.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 and rat heart, using CLCC1 Antibody.)

Immunofluorescene (IF)

(MBS9605386 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescene (IF) (MBS9605386 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from COLO cells using CLCC1 antibody., The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of extracts from COLO cells using CLCC1 antibody., The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-CLCC1 antibody
Function: Seems to act as a chloride ion channel.
Similarity: Belongs to the chloride channel MCLC family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 62 kD
Predicted: 63 kDa
NCBI Official Full Name
chloride channel CLIC-like protein 1 isoform 1
NCBI Official Synonym Full Names
chloride channel CLIC like 1
NCBI Official Symbol
CLCC1
NCBI Official Synonym Symbols
MCLC
NCBI Protein Information
chloride channel CLIC-like protein 1
UniProt Protein Name
Chloride channel CLIC-like protein 1
UniProt Gene Name
CLCC1
UniProt Synonym Gene Names
KIAA0761; MCLC

Uniprot Description

Seems to act as a chloride ion channel.

Research Articles on CLCC1

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Product Notes

The CLCC1 clcc1 (Catalog #AAA9605386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLCC1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CLCC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CLCC1 clcc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLCC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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