Rabbit CLC4 Polyclonal Antibody

CLC4 (Clcn4, Chloride Channel)

Cat. Num. AAA614443

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified over the peptide-Sepharose column
• Synonyms: CLC4; Polyclonal Antibody; CLC4 (Clcn4; Chloride Channel); Anti -CLC4 (Clcn4; anti-CLC4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The 13 AA CLC41-P control peptide is 100% conserved in mouse, rat and human CLC-4. No significant sequence homology is detected with other CLCs or other proteins. There is a very high degree of protein homology among CLC-3, CLC-4 and CLC-5. However, CLC41-P peptide has very little sequence homology with CLC-3 and CLC-5.
• Purity/Purification: Affinity Purified; Purified over the peptide-Sepharose column
• Form/Format: Supplied as a liquid in Tris, pH 7.5 and 0.2% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-CLC4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot:1-10ug/ml using ECL.; ELISA: 0.5-1ug/ml for affinity purified.
• Immunogen: A 13 AA Peptide sequence near the C-terminus of rat CLC-4 (1). The peptide was coupled to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CLC4 antibody

Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains.Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues.

Rat CLC-4 is 747 aa membrane protein (human CLC-4 760; mouse CLC-4 747 aa) (1). CLC-4 is abundant in liver, brain moderate levels are found in the heart, muscle, kidney, and spleen.

Product Categories/Family for anti-CLC4 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 5759112
• NCBI Official Full Name: chloride channel CLC4

NCBI Description

The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012]

Uniprot Description

CLCN4: Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Belongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily.

Protein type: Transporter; Membrane protein, integral; Transporter, ion channel; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: early endosome membrane; late endosome membrane; integral to membrane; endosome membrane

Molecular Function: chloride channel activity; voltage-gated chloride channel activity; ATP binding; antiporter activity

Biological Process: transport; chloride transport; transmembrane transport