Rabbit anti-Mouse Claudin 16 Polyclonal Antibody | anti-CLDN16 antibody

Claudin 16 (Paracellin 1, CLDN16)

Cat. Num. AAA630235

• Gene Names: CLDN16; HOMG3; PCLN1
• Reactivity: Mouse
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Claudin 16; Polyclonal Antibody; Claudin 16 (Paracellin 1; CLDN16); Anti -Claudin 16 (Paracellin 1; anti-CLDN16 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Claudin 16. Immunostaining of frozen mouse kidney sections localizes Claudin 16 to the thick ascending limb (TAL) of Henle's loop. This region was identi ed by an antibody against the Tamm-Horsfall protein, which is also localized to the TAL of Henle's loop in the kidney.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.01% sodium azide, before the addition of glycerol to 40.

• Applicable Applications for anti-CLDN16 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Dilution: Immuno uorescence: 1-3ug/ml; Immunohistochemistry (Frozen): 10ug/ml
• Immunogen: Synthetic peptide corresponding to the C-terminal region of human Claudin 16.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CLDN16 antibody

Claudin 16, also known as Paracellin-1, is a renal tight junction protein required for paracellular magnesium resorption. It is localized in the intercellular junctions of the epithelial cells of the thick ascending limb of Henle's loop (TAL), the distal tubule, and the collecting duct(6). Antibodies against Tamm-Horsfall protein (THP) (a TAL marker) are used to localize this specific structure of nephrons in kidney sections(1). Mg2+ resorption occurs in TAL(2), and Claudin 16 is required for this paracellular function(3). Failure in renal 'handling' of magnesium can result in hypermagnesemia, whose major direct toxicity implications are cardiovascular as well as renal failure. Recent studies have shown that the lack of Claudin-16 proteins contributes to the dysfunction of paracellular renal transport systems(5). Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of "molecular gasket" that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this so-called "barrier" function, the "fence" function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. Claudin proteins have been shown to be major integral membrane proteins localized at tight junctions.

Product Categories/Family for anti-CLDN16 antibody

Antibodies; Abs to Claudin

NCBI and Uniprot Product Information

• NCBI GI #: 5729970
• NCBI GeneID: 10686
• NCBI Accession #: NP_006571.1
• NCBI GenBank Nucleotide #: NM_006580.3
• UniProt Accession #: Q9Y5I7
• Molecular Weight: 33,836 Da
• NCBI Official Full Name: claudin-16
• NCBI Official Synonym Full Names: claudin 16
• NCBI Official Symbol: CLDN16
• NCBI Official Synonym Symbols: HOMG3; PCLN1
• NCBI Protein Information: claudin-16; PCLN-1; paracellin-1; OTTHUMP00000209499; hypomagnesemia 3, with hypercalciuria and nephrocalcinosis
• UniProt Protein Name: Claudin-16
• Protein Family: Claudin
• UniProt Gene Name: CLDN16
• UniProt Synonym Gene Names: PCLN1
• UniProt Entry Name: CLD16_HUMAN

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28. [provided by RefSeq]

Uniprot Description

Claudin-16: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Defects in CLDN16 are the cause of hypomagnesemia type 3 (HOMG3); also known as familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). HOMG3 is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis. Recurrent urinary tract infections and kidney stones are often observed. In spite of hypercalciuria, patients do not show hypocalcemia. Belongs to the claudin family.

Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: tight junction; plasma membrane; integral to membrane

Molecular Function: identical protein binding; protein binding; magnesium ion transmembrane transporter activity; structural molecule activity

Biological Process: cellular metal ion homeostasis; excretion; magnesium ion transport; calcium-independent cell-cell adhesion

Disease: Hypomagnesemia 3, Renal