Rabbit anti-Human Claudin 15 Polyclonal Antibody | anti-CLDN15 antibody

Claudin 15 Antibody

Cat. Num. AAA9629685

• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Claudin 15; Polyclonal Antibody; Claudin 15 Antibody; Claudin-15; CLD15_HUMAN; CLDN15; anti-CLDN15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Claudin 15 Antibody detects endogenous levels of total Claudin 15.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CLDN15 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the claudin family.
• Post Translational Modifications: Palmitoylated.
• Subunit Structure: Can form linear homooligomers in the membrane, giving rise to tight junction strand-like structures.
• Tissue Specificity: Detected in colon (at protein level).
• Function: Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN15 forms tight junctions that mediate the paracellular transport of small monovalent cations along a concentration gradient, due to selective permeability for Na(+), Li(+) and K(+) ions, but selects against Cl(-) ions. Plays an important role in paracellular Na(+) transport in the intestine and in Na(+) homeostasis. Required for normal Na(+)-dependent intestinal nutrient uptake.
• Note: Tight junctions form continuous circumferential cell-cell contacts at the borders of apical and lateral cell membranes that seal the intercellular space and show up as strand-like structures in electron microscopy.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CLDN15 antibody

Cell junction; Tight junction; Cell membrane; Multi-pass membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 297515455
• NCBI GeneID: 24146
• NCBI Accession #: NP_001172009.1
• NCBI GenBank Nucleotide #: NM_001185080.1
• UniProt Accession #: P56746; B3KPB5
• Molecular Weight: 24,356 Da
• NCBI Official Full Name: claudin-15
• NCBI Official Synonym Full Names: claudin 15
• NCBI Official Symbol: CLDN15
• NCBI Protein Information: claudin-15
• UniProt Protein Name: Claudin-15
• Protein Family: Claudin
• UniProt Gene Name: CLDN15

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN15 forms tight junctions that mediate the paracellular transport of small monovalent cations along a concentration gradient, due to selective permeability for Na+, Li+ and K+ ions, but selects against Cl- ions. Plays an important role in paracellular Na+ transport in the intestine and in Na+ homeostasis. Required for normal Na+-dependent intestinal nutrient uptake.