Rabbit anti-Human Claudin 1 Polyclonal Antibody | anti-CLDN1 antibody

Claudin 1 (Loop1) (Claudin-1, Senescence-associated Epithelial Membrane Protein, CLD1, SEMP1, CLDN1, UNQ481/PRO944) (MaxLight 490)

Cat. Num. AAA6469236

• Gene Names: CLDN1; CLD1; SEMP1; ILVASC
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Claudin 1; Polyclonal Antibody; Claudin 1 (Loop1) (Claudin-1; Senescence-associated Epithelial Membrane Protein; CLD1; SEMP1; CLDN1; UNQ481/PRO944) (MaxLight 490); anti-CLDN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLDN1.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-CLDN1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within aa40-82 of human Claudin 1, UniProt Accession #NP_066924; O95832.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A549 cell line lysates (35ug/lane) using C5838-02F. This demonstrates that C5838-02F detected the CLDN1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with C5838-02F, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of C5838-02F for immunohistochemistry.)

Product Categories/Family for anti-CLDN1 antibody

Antibodies; Claudin

References

1. Kinugasa, T., Anticancer Res. 27(6A), 3729-3734 (2007). 2. Paschoud, S., Mod. Pathol. 20(9), 947-954 (2007). 3. Morohashi, S., Int. J. Mol. Med. 20(2), 139-143 (2007). 4. Krajewska, M., Prostate 67(9), 907-910 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 10863887
• NCBI GeneID: 9076
• NCBI Accession #: NP_066924
• NCBI GenBank Nucleotide #: NM_021101
• UniProt Accession #: O95832
• Molecular Weight: 23kDa
• NCBI Official Full Name: claudin-1
• NCBI Official Synonym Full Names: claudin 1
• NCBI Official Symbol: CLDN1
• NCBI Official Synonym Symbols: CLD1; SEMP1; ILVASC
• NCBI Protein Information: claudin-1
• UniProt Protein Name: Claudin-1
• Protein Family: Claudin
• UniProt Gene Name: CLDN1
• UniProt Synonym Gene Names: CLD1; SEMP1
• UniProt Entry Name: CLD1_HUMAN

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

Claudin-1: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Acts as a co- receptor for HCV entry into hepatic cells. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL. May interact with HCV E1 and E2 proteins. Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. Belongs to the claudin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cytoskeletal

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: tight junction; integral to plasma membrane; apical plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: identical protein binding; protein binding; structural molecule activity

Biological Process: intercellular junction assembly and maintenance; viral reproduction; cell adhesion; calcium-independent cell-cell adhesion

Disease: Ichthyosis, Leukocyte Vacuoles, Alopecia, And Sclerosing Cholangitis