Rabbit anti-Human, Mouse CKMT2 Polyclonal Antibody | anti-CKMT2 antibody

CKMT2, NT (Creatine Kinase S-type, Mitochondrial, Sarcomeric Mitochondrial Creatine Kinase, S-MtCK, Basic-type Mitochondrial Creatine Kinase, Mib-CK) (Biotin)

Cat. Num. AAA6470525

• Gene Names: CKMT2; SMTCK
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CKMT2; Polyclonal Antibody; NT (Creatine Kinase S-type; Mitochondrial; Sarcomeric Mitochondrial Creatine Kinase; S-MtCK; Basic-type Mitochondrial Creatine Kinase; Mib-CK) (Biotin); anti-CKMT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CKMT2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CKMT2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CKMT2 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CKMT2 in human skeletal muscle and heart tissue lysates (35ug per lane) using MBS628186 at 1:1,000 dilution. Goat anti-rabbit IgG-HRP was used as secondary antibody.)

Product Categories/Family for anti-CKMT2 antibody

Antibodies; Enzymes; Creatine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 153251984
• NCBI GeneID: 1160
• NCBI Accession #: NP_001093205.1
• NCBI GenBank Nucleotide #: NM_001099735.1
• UniProt Accession #: P17540; Q6ICS8; Q8N1E1
• Molecular Weight: 47,504 Da
• NCBI Official Full Name: creatine kinase S-type, mitochondrial
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 2 (sarcomeric)
• NCBI Official Symbol: CKMT2
• NCBI Official Synonym Symbols: SMTCK
• NCBI Protein Information: creatine kinase S-type, mitochondrial; S-MtCK; mib-CK; basic-type mitochondrial creatine kinase; sarcomeric mitochondrial creatine kinase
• UniProt Protein Name: Creatine kinase S-type, mitochondrial
• Protein Family: Creatine kinase
• UniProt Gene Name: CKMT2
• UniProt Synonym Gene Names: Mib-CK; S-MtCK
• UniProt Entry Name: KCRS_HUMAN

NCBI Description

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CKMT2: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; Mitochondrial; Kinase, other; EC 2.7.3.2

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: creatine kinase activity; ATP binding

Biological Process: muscle contraction; creatine metabolic process; phosphorylation