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Western Blot (WB) (Western blot analysis of CKLF using Jurkat whole cell lysates)

Rabbit anti-Human, Mouse CKLF Polyclonal Antibody | anti-CKLF antibody

CKLF Antibody

Gene Names
CKLF; C32; CKLF1; CKLF2; CKLF3; CKLF4; UCK-1; HSPC224
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CKLF; Polyclonal Antibody; CKLF Antibody; C32; Chemokine-like factor; chemokine-like factor 1; chemokine-like factor 2; chemokine-like factor 3; chemokine-like factor 4; CKLF1; CKLF2; CKLF3; CKLF4; CKLFSF2; HSPC224; transmembrane proteolipid; UCK-1; UCK1; anti-CKLF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CKLF antibody detects endogenous levels of total CKLF
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
113
Applicable Applications for anti-CKLF antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
Immunogen
A synthesized peptide derived from human CKLF
Subcellular Location
Cell Membrane; Multipass Membrane Protein or Secreted
Tissue Specificity
Isoform 1, isoform 2, isoform 3 and isoform 4 have highest expression levels in adult spleen, lung, testis, ovary, peripheral blood leukocyte, placenta, pancreas, and in fetal brain, skeletal muscle, thymus and heart. Lower expression levels in adult skeletal muscle, liver, thymus colon, prostate and fetal spleen and liver.
Predicted Cross Reactivity
Horse
Similarity
Horse (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of CKLF using Jurkat whole cell lysates)

Western Blot (WB) (Western blot analysis of CKLF using Jurkat whole cell lysates)

Immunohistochemistry (IHC)

(MBS9609691 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9609691 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CKLF antibody
Function: May play an important role in inflammation and regeneration of skeletal muscle. Partly inhibited by interleukin 10.
Similarity: Belongs to the chemokine-like factor family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 17 kDa
Predicted: 18 kDa
NCBI Official Full Name
chemokine-like factor isoform e
NCBI Official Synonym Full Names
chemokine like factor
NCBI Official Symbol
CKLF
NCBI Official Synonym Symbols
C32; CKLF1; CKLF2; CKLF3; CKLF4; UCK-1; HSPC224
NCBI Protein Information
chemokine-like factor
UniProt Protein Name
Chemokine-like factor
Protein Family
UniProt Gene Name
CKLF
UniProt Synonym Gene Names
CKLF1

NCBI Description

The product of this gene is a cytokine. Cytokines are small proteins that have an essential role in the immune and inflammatory responses. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. The protein encoded by this gene is a potent chemoattractant for neutrophils, monocytes and lymphocytes. It also can stimulate the proliferation of skeletal muscle cells. This protein may play important roles in inflammation and in the regeneration of skeletal muscle. Alternatively spliced transcript variants encoding different isoforms have been identified. Naturally occurring read-through transcription occurs between this locus and the neighboring locus CMTM1 (CKLF-like MARVEL transmembrane domain containing 1).[provided by RefSeq, Feb 2011]

Uniprot Description

May play an important role in inflammation and regeneration of skeletal muscle. Partly inhibited by interleukin 10.

Research Articles on CKLF

Similar Products

Product Notes

The CKLF cklf (Catalog #AAA9609691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CKLF Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CKLF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the CKLF cklf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKLF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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