Rabbit anti-Human, Mouse CITED2 Polyclonal Antibody | anti-CITED2 antibody

CITED2, CT (CITED2, MRG1, Cbp/p300-interacting transactivator 2, MSG-related protein 1, P35srj) (PE)

Cat. Num. AAA6286227

• Gene Names: CITED2; ASD8; MRG1; VSD2; MRG-1; P35SRJ
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CITED2; Polyclonal Antibody; CT (CITED2; MRG1; Cbp/p300-interacting transactivator 2; MSG-related protein 1; P35srj) (PE); P35srj; anti-CITED2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CITED2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CITED2 antibody: Western Blot (WB), Immunofluorescence (IF), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 200-226 in the C-terminal region of human CITED2, UniProt Accession #NP_006070.2; Q99967; O35740; Q0VCT9. Species sequence homology: bovine
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane) usingMBS6012789. This demonstrates that MBS6012789 detected CITED2 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with Hela cells usingMBS6012789, followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CITED2 antibody

The protein encoded by this gene inhibits transactivation of HIF1A-induced genes by competing with binding of HIF1a to p300-CH1. Mutations in this gene are a cause of cardiac septal defects. Three transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-CITED2 antibody

Antibodies; Cardiac Markers

References

1. Yang, X.F., et al. Zhonghua Er Ke Za Zhi 48(4):293-296(2010). 2. Sun, H.B. Ann. N. Y. Acad. Sci. 1192, 429-436 (2010). 3. Lau, W.M., et al. Int. J. Cancer 126(4):876-884(2010). 4. van Agthoven, T., et al. Br. J. Cancer 101(11):1824-1832(2009). 5. Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 19923343
• NCBI GeneID: 10370
• NCBI Accession #: NP_006070.2
• NCBI GenBank Nucleotide #: NM_006079.4
• UniProt Accession #: Q99967; O35740; Q0VCT9
• Molecular Weight: 23,732 Da
• NCBI Official Full Name: cbp/p300-interacting transactivator 2 isoform 1
• NCBI Official Synonym Full Names: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
• NCBI Official Symbol: CITED2
• NCBI Official Synonym Symbols: ASD8; MRG1; VSD2; MRG-1; P35SRJ
• NCBI Protein Information: cbp/p300-interacting transactivator 2; MSG1-related gene 1; MSG-related protein 1; melanocyte-specific gene 1-related gene 1
• UniProt Protein Name: Cbp/p300-interacting transactivator 2
• Protein Family: Cbp/p300-interacting transactivator
• UniProt Gene Name: CITED2
• UniProt Synonym Gene Names: MRG1; MRG-1
• UniProt Entry Name: CITE2_HUMAN

NCBI Description

The protein encoded by this gene inhibits transactivation of HIF1A-induced genes by competing with binding of hypoxia-inducible factor 1-alpha to p300-CH1. Mutations in this gene are a cause of cardiac septal defects. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

CITED2: Transcriptional coactivator of the p300/CBP-mediated trancription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator- activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left- right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region. Defects in CITED2 are a cause of ventricular septal defect type 2 (VSD2). VSD2 is a common form of congenital cardiovascular anomaly that may occur alone or in combination with other cardiac malformations. It can affect any portion of the ventricular septum, resulting in abnormal communications between the two lower chambers of the heart. Classification is based on location of the communication, such as perimembranous, inlet, outlet (infundibular), central muscular, marginal muscular, or apical muscular defect. Large defects that go unrepaired may give rise to cardiac enlargement, congestive heart failure, pulmonary hypertension, Eisenmenger's syndrome, delayed fetal brain development, arrhythmias, and even sudden cardiac death. Defects in CITED2 are a cause of atrial septal defect type 8 (ASD8). ASD8 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Belongs to the CITED family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis; Apoptosis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: nuclear chromatin; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; protein binding; transcription coactivator activity; LBD domain binding; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: spleen development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; heart development; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell cycle; negative regulation of transcription from RNA polymerase II promoter; liver development; sex determination; cell proliferation; response to estrogen stimulus; response to hypoxia; positive regulation of cell-cell adhesion; positive regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; cell differentiation; negative regulation of transcription, DNA-dependent; negative regulation of cell migration; negative regulation of apoptosis

Disease: Atrial Septal Defect 8; Ventricular Septal Defect 2