Rabbit CISH Polyclonal Antibody | anti-CISH antibody

CISH Antibody

Cat. Num. AAA9609231

• Gene Names: CISH; CIS; G18; SOCS; CIS-1; BACTS2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CISH; Polyclonal Antibody; CISH Antibody; BACTS2; cis 1; CIS; CIS-1; CIS1; Cish; CISH_HUMAN; Cytokine inducible inhibitor of signaling type 1B; Cytokine inducible SH2 containing protein; Cytokine-inducible SH2-containing protein; G18; Protein G18; SOCS; Suppressor of cytokine signaling; anti-CISH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CISH antibody detects endogenous levels of total CISH
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-CISH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human CISH
• Tissue Specificity: Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
• Predicted Cross Reactivity: Bovine, Sheep, Rabbit, Dog
• Similarity: Bovine (83%), Sheep (83%), Rabbit (92%), Dog (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of CISH using Jurkat whole cell lysates)

Related Product Information for anti-CISH antibody

Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Subunit Structure: Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR).
Post-translational Modifications: Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).

NCBI and Uniprot Product Information

• NCBI GI #: 195976771
• NCBI GeneID: 1154
• NCBI Accession #: NP_037456.5
• NCBI GenBank Nucleotide #: NM_013324.5
• UniProt Accession #: Q9NSE2; Q9NS38; Q9Y5R1; B2R9N1; G5E9R1
• Molecular Weight: Observed: 29 kDa; Predicted: 29 kDa
• NCBI Official Full Name: cytokine-inducible SH2-containing protein isoform 1
• NCBI Official Synonym Full Names: cytokine inducible SH2 containing protein
• NCBI Official Symbol: CISH
• NCBI Official Synonym Symbols: CIS; G18; SOCS; CIS-1; BACTS2
• NCBI Protein Information: cytokine-inducible SH2-containing protein
• UniProt Protein Name: Cytokine-inducible SH2-containing protein
• Protein Family: Cytokine-inducible SH2-containing protein
• UniProt Gene Name: CISH
• UniProt Synonym Gene Names: G18; CIS; SOCS

NCBI Description

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins ().