Western Blot (WB)
(Western Blot (WB) analysis of CIDEB polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:The Spleen lysate of Mouse (40ug) Lane4:The Spleen lysate of Rat (40ug))
Rabbit CIDEB Polyclonal Antibody | anti-CIDEB antibody
CIDEB Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
CIDEB; Polyclonal Antibody; CIDEB Polyclonal Antibody; Cell death activator CIDE-B; Cell death-inducing DFFA-like effector B; anti-CIDEB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CIDEB polyclonal antibody detects endogenous levels of CIDEB protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
219
Applicable Applications for anti-CIDEB antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 104-148 of Human CIDEB
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of CIDEB polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:The Spleen lysate of Mouse (40ug) Lane4:The Spleen lysate of Rat (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of CIDEB polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:The Spleen lysate of Mouse (40ug) Lane4:The Spleen lysate of Rat (40ug))
Related Product Information for anti-CIDEB antibody
The DNA fragmentation factor (DFF) is involved in the caspase-3 apoptotic pathway. DFF is composed of two subunits, a 45 kDa subunit called DFF-45 (also designated ICAD, for inhibitor of CAD), and CPAN (caspase-activated nuclease), also designated CAD (caspase-activated deoxyribonuclease). CPAN is a DNase that is responsible for DNA degradation during apoptosis. CPAN is inhibited by DFF-45. Caspase-3 acts to dissociate CPAN from DFF-45, allowing CPAN to enter the nucleus and degrade DNA. CIDE-A and CIDE-B have been identified as proteins that share homology with the N-terminal region of DFF-45. Like CPAN, CIDE-A and CIDE-B promote cell death and DNA fragmentation and are inhibited by DFF-45.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 28kDa
NCBI Official Full Name
cell death activator CIDE-B
UniProt Protein Name
Cell death activator CIDE-B
Protein Family
UniProt Gene Name
CIDEB
UniProt Entry Name
CIDEB_HUMAN
Uniprot Description
CIDEB: Activates apoptosis.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: perinuclear region of cytoplasm; lipid particle; cytosol
Molecular Function: identical protein binding; protein binding
Biological Process: regulation of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis
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Product Notes
The CIDEB cideb (Catalog #AAA3007461) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CIDEB Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CIDEB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the CIDEB cideb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CIDEB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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