Rat Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) ELISA Kit | PIK3Cb elisa kit
Rat Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) ELISA Kit
Reactivity
Rat
Synonyms
Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb); N/A; Rat Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) ELISA Kit; PIK3Cb/Phosphoinositide-3-Kinase Catalytic Beta Polypeptide; PI3K; PI3Kbeta; PIK3C1; p110-BETA; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PIK3Cb elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of PIK3Cb. No significant cross-reactivity or interference between PIK3Cb and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Rat tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PIK3Cb were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PIK3Cb were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term(within shelf life)
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Related Product Information for PIK3Cb elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PIK3Cb. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PIK3Cb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIK3Cb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIK3Cb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIK3Cb in rat tissue homogenates, cell lysates and other biological fluids.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIK3Cb in rat tissue homogenates, cell lysates and other biological fluids.
Product Categories/Family for PIK3Cb elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
122,608 Da
NCBI Official Full Name
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
NCBI Official Synonym Full Names
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta
NCBI Official Symbol
Pik3cb
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3K; PI3Kbeta; p110beta; PI3K-beta; ptdIns-3-kinase p110; PI3-kinase subunit beta; PI3-kinase p110 subunit beta; ptdIns-3-kinase subunit beta; ptdIns-3-kinase subunit p110-bet
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
UniProt Gene Name
Pik3cb
UniProt Synonym Gene Names
PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta
UniProt Entry Name
PK3CB_RAT
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Product Notes
The Rat PIK3Cb pik3cb (Catalog #AAA151377) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA151377 ELISA Kit recognizes Rat PIK3Cb. It is sometimes possible for the material contained within the vial of "Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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