Rabbit anti-Human CHUK Polyclonal Antibody | anti-CHUK antibody

CHUK, CT (CHUK, IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha, Transcription factor 16)

Cat. Num. AAA647770

• Gene Names: CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CHUK; Polyclonal Antibody; CT (CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; Nuclear factor NF-kappa-B inhibitor kinase alpha; Transcription factor 16); Anti -CHUK; anti-CHUK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CHUK antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: CHUK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 554-582 amino acids from the C-terminal region of human CHUK.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHUK Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the CHUK antibody detected the CHUK protein (arrow).)

Related Product Information for anti-CHUK antibody

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Product Categories/Family for anti-CHUK antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 1589738
• NCBI GeneID: 1147
• UniProt Accession #: O15111; O14666; Q13132; Q5W0I4; Q92467
• Molecular Weight: 84,640 Da
• NCBI Official Full Name: CHUK, partial
• NCBI Official Synonym Full Names: conserved helix-loop-helix ubiquitous kinase
• NCBI Official Symbol: CHUK
• NCBI Official Synonym Symbols: IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit alpha; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: CHUK
• UniProt Synonym Gene Names: IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16
• UniProt Entry Name: IKKA_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Ref.17 Ref.22 Ref.25 Ref.27 Ref.29

Catalytic activity: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Enzyme regulation: Activated when phosphorylated and inactivated when dephosphorylated.

Subunit structure: Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Directly interacts with IKK-gamma/NEMO and TRPC4AP

By similarity. May interact with TRAF2. Interacts with NALP2. May interact with MAVS/IPS1. Interacts with ARRB1 and ARRB2. Interacts with NLRC5; prevents CHUK phosphorylation and kinase activity. Interacts with PIAS1; this interaction induces PIAS1 phosphorylation. Ref.9 Ref.15 Ref.18 Ref.19 Ref.20 Ref.21 Ref.25

Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and the nucleus. Ref.17

Tissue specificity: Widely expressed.

Domain: The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG. Ref.23

Post-translational modification: Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated. Ref.10 Ref.11 Ref.13 Ref.24Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

Involvement in disease: Cocoon syndrome (COCOS) [MIM:613630]: A lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.26

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.Contains 1 protein kinase domain.