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Western Blot (WB) (CHSY3 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CHSY3 antibody detected the CHSY3 protein (arrow).)

Rabbit CHSY3 Polyclonal Antibody | anti-CHSY3 antibody

CHSY3 Antibody (Center)

Gene Names
CHSY3; CSS3; CHSY2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CHSY3; Polyclonal Antibody; CHSY3 Antibody (Center); Chondroitin sulfate synthase 3; Carbohydrate synthase 2; Chondroitin glucuronyltransferase 3; Chondroitin synthase 2; ChSy-2; Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3; N-acetylgalactosaminyltransferase 3; CHSY2; CSS3; anti-CHSY3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CHSY3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human CHSY3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
531-560
Sequence Length
882
Applicable Applications for anti-CHSY3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(CHSY3 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CHSY3 antibody detected the CHSY3 protein (arrow).)

Western Blot (WB) (CHSY3 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CHSY3 antibody detected the CHSY3 protein (arrow).)
Related Product Information for anti-CHSY3 antibody
CSS3 is a glycosyltransferase that has both
glucuronyltransferase and N-acetylgalactosaminyltransferase
activities (Yada et al., 2003 [PubMed 12907687]).[supplied by
OMIM].
Product Categories/Family for anti-CHSY3 antibody
References
Izumikawa, T., et al. Biochem. J. 403(3):545-552(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Yada, T., et al. J. Biol. Chem. 278(41):39711-39725(2003)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
100284
NCBI Official Full Name
chondroitin sulfate synthase 3
NCBI Official Synonym Full Names
chondroitin sulfate synthase 3
NCBI Official Symbol
CHSY3
NCBI Official Synonym Symbols
CSS3; CHSY2
NCBI Protein Information
chondroitin sulfate synthase 3
UniProt Protein Name
Chondroitin sulfate synthase 3
UniProt Gene Name
CHSY3
UniProt Synonym Gene Names
CHSY2; CSS3; ChSy-2
UniProt Entry Name
CHSS3_HUMAN

NCBI Description

CSS3 is a glycosyltransferase that has both glucuronyltransferase and N-acetylgalactosaminyltransferase activities (Yada et al., 2003 [PubMed 12907687]).[supplied by OMIM, Mar 2008]

Uniprot Description

CSS3: Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1. Belongs to the chondroitin N- acetylgalactosaminyltransferase family.

Protein type: EC 2.4.1.175; EC 2.4.1.226; Transferase; Membrane protein, integral; Glycan Metabolism - chondroitin sulfate biosynthesis

Chromosomal Location of Human Ortholog: 5q23.3

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity; metal ion binding; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity

Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis

Research Articles on CHSY3

Similar Products

Product Notes

The CHSY3 chsy3 (Catalog #AAA9212648) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 531-560. The CHSY3 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's CHSY3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CHSY3 chsy3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHSY3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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