Rabbit anti-Human, Mouse CHST2 Polyclonal Antibody | anti-CHST2 antibody

CHST2, CT (CHST2, GN6ST, Carbohydrate sulfotransferase 2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2, N-acetylglucosamine 6-O-sulfotransferase 1) (MaxLight 405)

Cat. Num. AAA6285936

• Gene Names: CHST2; C6ST; GST2; GST-2; Gn6ST-1; glcNAc6ST-1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CHST2; Polyclonal Antibody; CT (CHST2; GN6ST; Carbohydrate sulfotransferase 2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; N-acetylglucosamine 6-O-sulfotransferase 1) (MaxLight 405); N-acetylglucosamine 6-O-sulfotransferase 1; anti-CHST2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHST2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-CHST2 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 493-522 in the C-terminal region of human CHST2.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in human placenta tissue lysates (35ug/lane) usingMBS646242. This demonstrates that MBS646242 detected the CHST2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse cerebellum tissue lysates (35ug/lane) usingMBS646242. This demonstrates that MBS646242 detected the CHST2 protein (arrow).)

Related Product Information for anti-CHST2 antibody

N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).

Product Categories/Family for anti-CHST2 antibody

Antibodies; Enzymes; Sulfotransferase

References

1. Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). 2. Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009). 3. Desko, M.M., et al. Glycobiology 19(10):1068-1077(2009). 4. Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009). 5. Kanoh, A., et al. Glycoconj. J. 23 (5-6), 453-460 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 27369497
• NCBI GeneID: 9435
• NCBI Accession #: NP_004258.2
• NCBI GenBank Nucleotide #: NM_004267.4
• UniProt Accession #: Q9Y4C5; Q2M370; Q9GZN5; Q9UED5; Q9Y6F2; D3DNG5
• Molecular Weight: 57,857 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 2
• NCBI Official Synonym Full Names: carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
• NCBI Official Symbol: CHST2
• NCBI Official Synonym Symbols: C6ST; GST2; GST-2; Gn6ST-1; glcNAc6ST-1
• NCBI Protein Information: carbohydrate sulfotransferase 2; N-acetylglucosamine 6-O-sulfotransferase 1; carbohydrate (chondroitin 6/keratan) sulfotransferase 2; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2
• UniProt Protein Name: Carbohydrate sulfotransferase 2
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST2
• UniProt Synonym Gene Names: GN6ST; GST-2; GlcNAc6ST-1; Gn6ST-1
• UniProt Entry Name: CHST2_HUMAN

NCBI Description

This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]

Uniprot Description

CHST2: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Transferase; EC 2.8.2.-; Glycan Metabolism - keratan sulfate biosynthesis

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: Golgi membrane; intrinsic to Golgi membrane; integral to membrane; trans-Golgi network

Molecular Function: sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; multicellular organismal development; carbohydrate metabolic process; keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; pathogenesis; inflammatory response