Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Chromatin Assembly Factor 1 Polyclonal Antibody | anti-CHAF1B antibody
Polyclonal Antibody to Chromatin Assembly Factor 1, Subunit B (CHAF1B)
Gene Names
Chaf1b; CAF1; CAF1A; C76145; CAF1P60; CAF-Ip60; MPHOSPH7; CAF-I p60; 2600017H24Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Chromatin Assembly Factor 1; Polyclonal Antibody; Polyclonal Antibody to Chromatin Assembly Factor 1; Subunit B (CHAF1B); anti-CHAF1B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CHAF1B. It has beenselected for its ability to recognize CHAF1B in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
559
Applicable Applications for anti-CHAF1B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CHAF1B (Met1~Asn199)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant CHAF1B (Met1~Asn199) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081320
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63,132 Da
NCBI Official Full Name
chromatin assembly factor 1 subunit B
NCBI Official Synonym Full Names
chromatin assembly factor 1, subunit B (p60)
NCBI Official Symbol
Chaf1b
NCBI Official Synonym Symbols
CAF1; CAF1A; C76145; CAF1P60; CAF-Ip60; MPHOSPH7; CAF-I p60; 2600017H24Rik
NCBI Protein Information
chromatin assembly factor 1 subunit B
UniProt Protein Name
Chromatin assembly factor 1 subunit B
Protein Family
UniProt Gene Name
Chaf1b
UniProt Synonym Gene Names
CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60
Uniprot Description
CAF-1B: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the WD repeat HIR1 family.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 16 C4|16 54.96 cM
Cellular Component: cytosol; nuclear chromatin; nucleoplasm; nucleus; protein complex
Molecular Function: histone binding; protein binding
Biological Process: cell cycle; cellular response to DNA damage stimulus; chromatin assembly; DNA repair; DNA replication; DNA replication-dependent nucleosome assembly; nucleosome assembly; regulation of transcription, DNA-templated; transcription, DNA-dependent
Similar Products
Product Notes
The CHAF1B chaf1b (Catalog #AAA2027443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Chromatin Assembly Factor 1, Subunit B (CHAF1B) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Chromatin Assembly Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CHAF1B chaf1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chromatin Assembly Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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