Rabbit CHRNB2 Polyclonal Antibody | anti-CHRNB2 antibody

CHRNB2 Antibody (N-Term)

Cat. Num. AAA9216451

• Gene Names: CHRNB2; EFNL3; nAChRB2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: CHRNB2; Polyclonal Antibody; CHRNB2 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Neuronal acetylcholine receptor subunit beta-2; anti-CHRNB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This CHRNB2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 66-100 amino acids from human CHRNB2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 502

• Applicable Applications for anti-CHRNB2 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-CHRNB2 Antibody (N-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: mouse liver lysateLane 3: rat testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-CHRNB2 antibody

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions.

Product Categories/Family for anti-CHRNB2 antibody

Cancer; Metabolism; Neuroscience

References

Anand R.,et al.Nucleic Acids Res. 18:4272-4272(1990). Elliott K.J.,et al.J. Mol. Neurosci. 7:217-228(1996). Groot Kormelink P.J.,et al.FEBS Lett. 400:309-314(1997). Rempel N.,et al.Hum. Genet. 103:645-653(1998). Lueders K.K.,et al.Mamm. Genome 10:900-905(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4502833
• NCBI GeneID: 1141
• NCBI Accession #: NP_000739.1
• NCBI GenBank Nucleotide #: NM_000748.2
• UniProt Accession #: P17787; Q9UEH9
• Molecular Weight: 57019
• NCBI Official Full Name: neuronal acetylcholine receptor subunit beta-2
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic beta 2
• NCBI Official Symbol: CHRNB2
• NCBI Official Synonym Symbols: EFNL3; nAChRB2
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-2
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-2
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNB2
• UniProt Entry Name: ACHB2_HUMAN

NCBI Description

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]

Uniprot Description

nAChRB2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3). ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 2/CHRNB2 sub-subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.; Channel, ligand-gated; Channel, cation

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; neuron projection; integral to membrane; plasma membrane; synapse; cell junction; external side of plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; acetylcholine receptor activity; drug binding; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding; ligand-gated ion channel activity

Biological Process: response to nicotine; positive regulation of dopamine secretion; protein heterooligomerization; regulation of dendrite morphogenesis; behavioral response to nicotine; locomotory behavior; regulation of circadian sleep/wake cycle, non-REM sleep; signal transduction; sensory perception of pain; regulation of dopamine secretion; synaptic transmission, cholinergic; synaptic transmission; smooth muscle contraction; sensory perception of sound; regulation of synaptic transmission, dopaminergic; visual perception; calcium ion transport; conditioned taste aversion; positive regulation of B cell proliferation; visual learning; synaptic transmission involved in micturition; associative learning; regulation of circadian sleep/wake cycle, REM sleep; vestibulocochlear nerve development; regulation of action potential; B cell activation; regulation of dopamine metabolic process; positive regulation of synaptic transmission, dopaminergic; central nervous system projection neuron axonogenesis; learning; social behavior; negative regulation of action potential; optic nerve morphogenesis; response to cocaine; memory; neurological system process; membrane depolarization; regulation of membrane potential; response to ethanol; regulation of synaptogenesis; response to hypoxia; neuromuscular synaptic transmission; ion transport; cognition; lateral geniculate nucleus development

Disease: Epilepsy, Nocturnal Frontal Lobe, 3