Goat CHRNB2 Polyclonal Antibody | anti-CHRNB2 antibody

Goat Polyclonal to Human CHRNB2

Cat. Num. AAA244691

• Gene Names: CHRNB2; EFNL3; nAChRB2
• Reactivity: Bat, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CHRNB2; Polyclonal Antibody; Goat Polyclonal to Human CHRNB2; Anti-CHRNB2 Antibody (Internal) IHC-plus; AChR-beta1; Acrb2; EFNL3; NAChR beta 2; NAChRB2; Human CHRNB2; anti-CHRNB2 antibody

• Host: Goat
• Reactivity: Bat, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Pig (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CHRNB2.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 502

• Applicable Applications for anti-CHRNB2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 48 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex) lysates (calculated MW of 57.0 kDa according to NP_000739.1). The observed molecular weight corresponds...
• Target Species: Human
• Immunogen Description: Synthetic peptide QPRHHCARQRLR from an internal region of human CHRNB2 (NP_000739.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Elephant, Bat (100%); Mouse, Rat, Hamster, Bovine, Dog, Pig, Opossum (92%); Ferret, Xenopus (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: CHRNB2 antibody was raised against synthetic peptide QPRHHCARQRLR from an internal region of human CHRNB2 (NP_000739.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Elephant, Bat (100%); Mouse, Rat, Hamster, Bovine, Dog, Pig, Opossum (92%); Ferret, Xenopus (83%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CHRNB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)

Western Blot (WB)

(CHRNB2 antibody (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-CHRNB2 antibody

Family: Ion Channel; Subfamily: Nicotinic acetylcholine receptor

NCBI and Uniprot Product Information

• NCBI GI #: 4502833
• NCBI GeneID: 1141
• NCBI Accession #: NP_000739.1
• NCBI GenBank Nucleotide #: NM_000748.2
• UniProt Accession #: P17787; Q9UEH9
• Molecular Weight: 57,019 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit beta-2
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, beta 2 (neuronal)
• NCBI Official Symbol: CHRNB2
• NCBI Official Synonym Symbols: EFNL3; nAChRB2
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-2; neuronal nicotinic acetylcholine receptor beta 2; acetylcholine receptor, nicotinic, beta 2 (neuronal); beta2 human neuronal nicotinic acetylcholine receptor; cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-2
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNB2
• UniProt Entry Name: ACHB2_HUMAN

NCBI Description

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]

Uniprot Description

nAChRB2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3). ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 2/CHRNB2 sub-subfamily.

Protein type: Channel, cation; Receptor, misc.; Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; integral to membrane; plasma membrane; cell junction; external side of plasma membrane

Molecular Function: protein heterodimerization activity; acetylcholine receptor activity; drug binding; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding; ligand-gated ion channel activity

Biological Process: response to nicotine; positive regulation of dopamine secretion; protein heterooligomerization; regulation of dendrite morphogenesis; behavioral response to nicotine; locomotory behavior; regulation of circadian sleep/wake cycle, non-REM sleep; sensory perception of pain; signal transduction; regulation of dopamine secretion; synaptic transmission, cholinergic; synaptic transmission; regulation of synaptic transmission, dopaminergic; smooth muscle contraction; sensory perception of sound; visual perception; conditioned taste aversion; calcium ion transport; positive regulation of B cell proliferation; visual learning; synaptic transmission involved in micturition; associative learning; regulation of circadian sleep/wake cycle, REM sleep; vestibulocochlear nerve development; regulation of action potential; B cell activation; regulation of dopamine metabolic process; positive regulation of synaptic transmission, dopaminergic; central nervous system projection neuron axonogenesis; negative regulation of action potential; learning; social behavior; optic nerve morphogenesis; response to cocaine; memory; neurological system process; membrane depolarization; response to ethanol; regulation of synaptogenesis; response to hypoxia; ion transport; cognition; lateral geniculate nucleus development

Disease: Epilepsy, Nocturnal Frontal Lobe, 3