Rabbit CHRNA7 Polyclonal Antibody | anti-CHRNA7 antibody

CHRNA7 Rabbit pAb

Cat. Num. AAA9459317

• Gene Names: CHRFAM7A; D-10; CHRNA7; CHRNA7-DR1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: CHRNA7; Polyclonal Antibody; CHRNA7 Rabbit pAb; CHRNA7-2; NACHRA7; anti-CHRNA7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-CHRNA7 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant fusion protein of human CHRNA7 (NP_001177384.1).
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using CHRNA7 at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using CHRNA7 at dilution of 1:100 (40x lens).)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CHRNA7 at 1:1000 dilution.)

Related Product Information for anti-CHRNA7 antibody

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-CHRNA7 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 2506127
• NCBI GeneID: 89832
• UniProt Accession #: P36544; Q15826; Q8IUZ4; Q96RH2; Q99555; Q9BXH0; A8K7Q4; B4DFS0; Q494W8
• Molecular Weight: 502
• NCBI Official Full Name: Neuronal acetylcholine receptor subunit alpha-7
• NCBI Official Synonym Full Names: CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
• NCBI Official Symbol: CHRFAM7A
• NCBI Official Synonym Symbols: D-10; CHRNA7; CHRNA7-DR1
• NCBI Protein Information: CHRNA7-FAM7A fusion protein; alpha-7 nicotinic cholinergic receptor subunit; alpha 7 neuronal nicotinic acetylcholine receptor-FAM7A hybrid; CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A (family with sequence similari
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-7
• Protein Family: CHRNA7-FAM7A fusion protein
• UniProt Gene Name: CHRNA7
• UniProt Synonym Gene Names: NACHRA7
• UniProt Entry Name: ACHA7_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7, which is located on chromosome 15 in a region associated with several neuropsychiatric disorders, is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene, translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed. [provided by RefSeq, Jul 2008]

Uniprot Description

nAChRA7: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 7/CHRNA7 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; plasma membrane; integral to membrane; cell junction

Molecular Function: toxin binding; chloride channel regulator activity; protein homodimerization activity; beta-amyloid binding; acetylcholine-gated cation channel activity; acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding

Biological Process: response to nicotine; cellular calcium ion homeostasis; synaptic transmission; positive regulation of angiogenesis; activation of MAPK activity; calcium ion transport; positive regulation of cell proliferation; response to hypoxia; ion transport; negative regulation of tumor necrosis factor production; signal transduction; cognition; memory

Disease: Chromosome 15q13.3 Deletion Syndrome