Rabbit anti-Human CHRDL1 Polyclonal Antibody | anti-CHRDL1 antibody

CHRDL1, NT (CHRDL1, NRLN1, Chordin-like protein 1, Neuralin-1, Neurogenesin-1, Ventroptin) (FITC)

Cat. Num. AAA6285802

• Gene Names: CHRDL1; CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHRDL1; Polyclonal Antibody; NT (CHRDL1; NRLN1; Chordin-like protein 1; Neuralin-1; Neurogenesin-1; Ventroptin) (FITC); Ventroptin; anti-CHRDL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 456

• Applicable Applications for anti-CHRDL1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CHRDL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of human CHRDL1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHRDL1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHRDL1 antibody detected the CHRDL1 protein (arrow).)

Immunohistochemistry (IHC)

(CHRDL1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRDL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CHRDL1 antibody

CHRDL1 is an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Categories/Family for anti-CHRDL1 antibody

Antibodies; Stem Cell Research

References

Fernandes, H., et al. Cells Tissues Organs (Print) 191(6):443-452(2010). Larman, B.W., et al. J. Am. Soc. Nephrol. 20(5):1020-1031(2009). Kane, R., et al. Mol. Vis. 14, 1138-1148 (2008): Kosinski, C., et al. Proc. Natl. Acad. Sci. U.S.A. 104(39):15418-15423(2007). Gazzerro, E., et al. Rev Endocr Metab Disord 7 (1-2), 51-65 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 34147715
• NCBI GeneID: 91851
• NCBI Accession #: NP_660277
• NCBI GenBank Nucleotide #: NP_660277.2
• UniProt Accession #: Q9BU40
• NCBI Official Full Name: chordin-like protein 1 isoform 3
• NCBI Official Synonym Full Names: chordin like 1
• NCBI Official Symbol: CHRDL1
• NCBI Official Synonym Symbols: CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
• NCBI Protein Information: chordin-like protein 1
• UniProt Protein Name: Chordin-like protein 1
• Protein Family: Chordin-like protein
• UniProt Gene Name: CHRDL1
• UniProt Synonym Gene Names: NRLN1

NCBI Description

This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation (). May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.