Rabbit anti-Human Chorionic Gonadotropin, beta Polyclonal Antibody | anti-CGB antibody

Chorionic Gonadotropin, Human, beta CT (CGB, CGB3, Choriogonadotropin subunit beta, Chorionic gonadotrophin chain beta) (Azide free) (HRP)

Cat. Num. AAA6285748

• Gene Names: CGB7; CGB6; CG-beta-a
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Chorionic Gonadotropin; beta; Polyclonal Antibody; Human; beta CT (CGB; CGB3; Choriogonadotropin subunit beta; Chorionic gonadotrophin chain beta) (Azide free) (HRP); CGB; Chorionic gonadotrophin chain beta; anti-CGB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CGB antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CGB/HCG-Beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-165 amino acids from the C-terminal region of human CGB/HCG-Alpha/Beta.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CGB/HCG-Beta Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the CGB/HCG-Beta antibody detected the CGB/HCG-Beta protein (arrow).)

Immunohistochemistry (IHC)

(CGB/HCG-Beta Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CGB/HCG-Beta Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CGB antibody

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene.

Product Categories/Family for anti-CGB antibody

Antibodies; Hormones; Steroids

References

Cole, L.A. Placenta 31(8):653-664(2010). Ra, Y.J., et al. Interact Cardiovasc Thorac Surg 11(1):114-116(2010). Verma, B., et al. J. Immunol. 184(4):2156-2165(2010). Handschuh, K., et al. Placenta 30(12):1016-1022(2009). Reisenbichler, E.S., et al. Breast J 15(5):527-530(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 15451750
• NCBI GeneID: 94027
• NCBI Accession #: NP_149133.1
• NCBI GenBank Nucleotide #: NM_033142.1
• UniProt Accession #: P01233
• NCBI Official Full Name: chorionic gonadotropin, beta polypeptide 7
• NCBI Official Synonym Full Names: chorionic gonadotropin, beta polypeptide 7
• NCBI Official Symbol: CGB7
• NCBI Official Synonym Symbols: CGB6; CG-beta-a
• NCBI Protein Information: chorionic gonadotropin, beta polypeptide 7; chorionic gonadotropin beta 7 subunit
• UniProt Protein Name: Choriogonadotropin subunit beta
• Protein Family: Choriogonadotropin
• UniProt Gene Name: CGB
• UniProt Synonym Gene Names: CGB3; CG-beta
• UniProt Entry Name: CGHB_HUMAN

NCBI Description

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 7 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CGB: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Belongs to the glycoprotein hormones subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: cellular protein metabolic process; cell-cell signaling; apoptosis; peptide hormone processing; female gamete generation; signal transduction