Rabbit Chondroitin Sulfate Proteoglycan Polyclonal Antibody | anti-CSPG antibody

Chondroitin Sulfate Proteoglycan (NG2, CSPG) (Biotin)

Cat. Num. AAA6468855

• Gene Names: CSPG4; NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG
• Reactivity: Rat, Human, Monkey, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Chondroitin Sulfate Proteoglycan; Polyclonal Antibody; Chondroitin Sulfate Proteoglycan (NG2; CSPG) (Biotin); anti-CSPG antibody

• Host: Rabbit
• Reactivity: Rat, Human, Monkey, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat NG2 Chondroitin Sulfate Proteoglycan between ~270-300kD. Reacts with both the intact Chondroitin Sulfate Proteoglycan and the core protein by Western Blot and ELISA. When oligodendrocyte pecurser cells (O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. Does not stain diferentatied oligodendrocytes well. Species Crossreactivity: human, mouse and monkey.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 8305

• Applicable Applications for anti-CSPG antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: ELISA: 1:1500-1:3000; WB: 1:1000. Detects NG2 chondroitin sulfate on 10ug of rat brain lysates.; ICC: 1:150-1:600; IHC: 1:200 on frozen embryonic mouse brain tissue using an Alexa Fluor labeled secondary antibody. It is suggested that the tissue used is only lightly fixed (2-4% paraformaldehyde, 5-10min). Avoid overfixing tissue.; IP: 2ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Immunoaffinity purified rat NG2 Chondroitin Sulfate Proteoglycan (NM_001897.4).
• Conjugate: Biotin
• Positive Control: Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot Analysis: Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with C5067-70D (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.)

Testing Data

(Rat Brain (Lane 1) and Mouse Brain Membrane were resolved by electrophoresis, transferred to PVDF membrane and probed with C5067-70D (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.)

Testing Data

(C5067-70D staining of adult rat hippocampus. Photo shows single oligodendrocyte precursor cell.)

Testing Data

(C5067-70D staining of adult rat cortex. Photo shows oligodendrocyte precursor cell.)

Related Product Information for anti-CSPG antibody

NG2 is a high molecular weight, integral membrane chondroitin sufate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet derived growth factor.

Product Categories/Family for anti-CSPG antibody

Antibodies; Carbohydrates; Glycoproteins

References

1. Rolls, A., et al., Nat. Cell Biol. 9: 1081-1088 (2007). 2. Ishibashi, T., et al., Neuron 49(6): 823-832 (2006). 3. Ponti, G., et al., Dev. Biol. 294: 168-180 (2006). 4. Wicher, G., et al., J. Neurosci. Res. 83: 864-873 (2006). 5. Zhao, L., et al., J. Neurosci. 26: 11, 278-11, 286 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 126091140
• NCBI GeneID: 1464
• NCBI Accession #: NM_001897.4
• NCBI GenBank Nucleotide #: NM_001897.4
• UniProt Accession #: Q6UVK1
• NCBI Official Full Name: Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA
• NCBI Official Synonym Full Names: chondroitin sulfate proteoglycan 4
• NCBI Official Symbol: CSPG4
• NCBI Official Synonym Symbols: NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG
• NCBI Protein Information: chondroitin sulfate proteoglycan 4
• UniProt Protein Name: Chondroitin sulfate proteoglycan 4
• Protein Family: Cold shock-like protein
• UniProt Gene Name: CSPG4
• UniProt Synonym Gene Names: MCSP
• UniProt Entry Name: CSPG4_HUMAN

NCBI Description

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Uniprot Description

NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: lysosomal lumen; cell surface; focal adhesion; integral to plasma membrane; Golgi lumen; apical plasma membrane; extracellular region

Molecular Function: signal transducer activity; protein kinase binding

Biological Process: chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; activation of MAPK activity; tissue remodeling; pathogenesis; glial cell migration; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; carbohydrate metabolic process; chondroitin sulfate catabolic process; angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway