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Western Blot (WB) (Western Blot: Sample: Rat Brain Tissue.)

Rabbit anti-Rat Cholinergic Receptor, Muscarinic 1 (CHRM1) Polyclonal Antibody | anti-CHRM1 antibody

Polyclonal Antibody to Cholinergic Receptor, Muscarinic 1 (CHRM1)

Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Cholinergic Receptor; Muscarinic 1 (CHRM1); Polyclonal Antibody; Polyclonal Antibody to Cholinergic Receptor; anti-CHRM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CHRM1. It has been selected for its ability to recognize CHRM1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.77mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- R IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA KRKTFSLVKE KKA
Sequence Length
460
Applicable Applications for anti-CHRM1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CHRM1 (Arg210~Ala363) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2050238
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Brain Tissue.)

Western Blot (WB) (Western Blot: Sample: Rat Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant CHRM1, Rat.)

Western Blot (WB) (Western Blot: Sample: Recombinant CHRM1, Rat.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Kidney Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Kidney Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Brain Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Brain Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Stomach Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Stomach Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,369 Da
NCBI Official Full Name
muscarinic acetylcholine receptor M1
NCBI Official Synonym Full Names
cholinergic receptor, muscarinic 1
NCBI Official Symbol
Chrm1
NCBI Protein Information
muscarinic acetylcholine receptor M1
UniProt Protein Name
Muscarinic acetylcholine receptor M1
UniProt Gene Name
Chrm1
UniProt Synonym Gene Names
Chrm-1

NCBI Description

plays a role in acetylcholine receptor signaling and neuromuscular synaptic transmission [RGD, Feb 2006]

Uniprot Description

mAChR m1: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: asymmetric synapse; axon terminus; cell junction; dendrite; integral component of plasma membrane; plasma membrane; postsynaptic density; postsynaptic membrane; synapse

Molecular Function: drug binding; G-protein coupled acetylcholine receptor activity

Biological Process: cognition; G-protein coupled acetylcholine receptor signaling pathway; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; muscarinic acetylcholine receptor, phospholipase C activating pathway; neuromuscular synaptic transmission; positive regulation of ion transport; regulation of locomotion; regulation of vascular smooth muscle contraction; saliva secretion; synaptic transmission, cholinergic

Research Articles on CHRM1

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Product Notes

The CHRM1 chrm1 (Catalog #AAA2006991) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cholinergic Receptor, Muscarinic 1 (CHRM1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cholinergic Receptor, Muscarinic 1 (CHRM1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CHRM1 chrm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- R IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA KRKTFSLVKE KKA. It is sometimes possible for the material contained within the vial of "Cholinergic Receptor, Muscarinic 1 (CHRM1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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