Rabbit anti-Human, Mouse Choline Acetyltransferase Polyclonal Antibody | anti-CHAT antibody

Rabbit Anti-Human Choline Acetyltransferase Polyclonal

Cat. Num. AAA807689

• Gene Names: CHAT; CMS6; CMS1A; CMS1A2; CHOACTASE
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: Choline Acetyltransferase; Polyclonal Antibody; Rabbit Anti-Human Choline Acetyltransferase Polyclonal; CHAT_Human Antibody; Acetyl CoA choline O Acetyltransferase Antibody; Choline Acetylase Antibody; CLAT_Human Antibody; CMS1A Antibody; Choline Acetyltransferase Antibody: RPE ; Choline Acetyltransferase Antibody; anti-CHAT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 630

• Applicable Applications for anti-CHAT antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
• Immunogen Species: Human
• COA: A 1:1000 dilution was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Conjugate: RPE
• Cellular Localization: Cytoplasm, Cytosol, Mitochondrion, Nucleus
• Preparation and Storage: Store at -20 degree C

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite.)

Western Blot (WB)

(Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa.)

Related Product Information for anti-CHAT antibody

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).

Product Categories/Family for anti-CHAT antibody

Neuroscience; Neurotransmitter Receptors; Cell Signaling; Neurodegeneration; Alzheimer's Disease

References

1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266(1): 97-119. 2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.

NCBI and Uniprot Product Information

• NCBI GI #: 218931221
• NCBI GeneID: 1103
• NCBI Accession #: NP_001136401.1
• NCBI GenBank Nucleotide #: NM_001142929.1
• UniProt Accession #: P28329; Q16488; Q9BQ23; Q9BQ35; Q9BQE1; A2BDF4; A2BDF5
• Molecular Weight: Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.
• NCBI Official Full Name: choline O-acetyltransferase isoform 1
• NCBI Official Synonym Full Names: choline O-acetyltransferase
• NCBI Official Symbol: CHAT
• NCBI Official Synonym Symbols: CMS6; CMS1A; CMS1A2; CHOACTASE
• NCBI Protein Information: choline O-acetyltransferase
• UniProt Protein Name: Choline O-acetyltransferase
• Protein Family: (Z)-3-hexen-1-ol acetyltransferase
• UniProt Gene Name: CHAT
• UniProt Synonym Gene Names: CHOACTase; ChAT; Choline acetylase
• UniProt Entry Name: CLAT_HUMAN

NCBI Description

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

Uniprot Description

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: EC 2.3.1.6; Lipid Metabolism - glycerophospholipid; Acetyltransferase

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cytoplasm; cytosol; mitochondrion; neuron projection; nucleus

Molecular Function: choline binding; choline O-acetyltransferase activity

Biological Process: acetylcholine biosynthetic process; antral ovarian follicle growth; glycerophospholipid biosynthetic process; memory; neurotransmitter secretion; phosphatidylcholine biosynthetic process; phospholipid metabolic process; response to drug; response to ethanol; response to hypoxia; response to nutrient; synaptic transmission

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea