Rabbit anti-Human CHMP5 Polyclonal Antibody | anti-CHMP5 antibody

CHMP5, CT (CHMP5, C9orf83, SNF7DC2, Charged multivesicular body protein 5, Chromatin-modifying protein 5, SNF7 domain-containing protein 2, Vacuolar protein sorting-associated protein 60) (MaxLight 750)

Cat. Num. AAA6285698

• Gene Names: CHMP5; Vps60; CGI-34; PNAS-2; C9orf83; HSPC177; SNF7DC2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CHMP5; Polyclonal Antibody; CT (CHMP5; C9orf83; SNF7DC2; Charged multivesicular body protein 5; Chromatin-modifying protein 5; SNF7 domain-containing protein 2; Vacuolar protein sorting-associated protein 60) (MaxLight 750); Vacuolar protein sorting-associated protein 60; anti-CHMP5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHMP5.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-CHMP5 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 178-204 in the C-terminal region of human CHMP5, UniProt Accession #NP_057494.3; Q9NZZ3; Q4QQV8; Q9D7S9. Species sequence homology: mouse, rat
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6010821. This demonstrates that MBS6010821 detected the CHMP5 protein (arrow).)

Related Product Information for anti-CHMP5 antibody

CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).

Product Categories/Family for anti-CHMP5 antibody

Antibodies; Chromatin Proteins

References

1. Wang, H.R., et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi 16(2):282-285(2008). 2. Row, P.E., et al. J. Biol. Chem. 282(42):30929-30937(2007). 3. Huang, H.H., et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi 15(4):738-742(2007). 4. Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007). 5. Wang, H.R., et al. Oncology 71 (5-6), 423-429 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 189409150
• NCBI GeneID: 51510
• NCBI Accession #: NP_057494.3
• NCBI GenBank Nucleotide #: NM_016410.5
• UniProt Accession #: Q9NZZ3; Q4QQV8; Q9D7S9
• Molecular Weight: 19,520 Da
• NCBI Official Full Name: charged multivesicular body protein 5 isoform 1
• NCBI Official Synonym Full Names: charged multivesicular body protein 5
• NCBI Official Symbol: CHMP5
• NCBI Official Synonym Symbols: Vps60; CGI-34; PNAS-2; C9orf83; HSPC177; SNF7DC2
• NCBI Protein Information: charged multivesicular body protein 5
• UniProt Protein Name: Charged multivesicular body protein 5
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP5
• UniProt Synonym Gene Names: C9orf83; SNF7DC2; Vps60; hVps60
• UniProt Entry Name: CHMP5_HUMAN

NCBI Description

CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]

Uniprot Description

CHMP5: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. Belongs to the SNF7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: endosome membrane; nucleus; cytosol

Molecular Function: protein binding

Biological Process: protein transport; viral reproduction; cell separation during cytokinesis; lysosome organization and biogenesis; regulation of receptor recycling; endosome to lysosome transport; viral infectious cycle; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis