Rabbit anti-Human CHMP4B Polyclonal Antibody | anti-CHMP4B antibody

CHMP4B, NT (CHMP4B, C20orf178, SHAX1, Charged multivesicular body protein 4b, Chromatin-modifying protein 4b, SNF7 homolog associated with Alix 1, SNF7-2, Vacuolar protein sorting-associated protein 32-2) (FITC)

Cat. Num. AAA6285681

• Gene Names: CHMP4B; SNF7; CTPP3; Shax1; CHMP4A; SNF7-2; VPS32B; CTRCT31; Vps32-2; C20orf178; dJ553F4.4
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHMP4B; Polyclonal Antibody; NT (CHMP4B; C20orf178; SHAX1; Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; SNF7 homolog associated with Alix 1; SNF7-2; Vacuolar protein sorting-associated protein 32-2) (FITC); Vacuolar protein sorting-associated protein 32-2; anti-CHMP4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CHMP4B antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CHMP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~87 amino acids from the C-terminal region of human DIO3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CHMP4B Antibody (N-term) in SK-BR-3 cell line lysates (35ug/lane).CHMP4B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CHMP4B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CHMP4B Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CHMP4B antibody

CHMP4B is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies.

Product Categories/Family for anti-CHMP4B antibody

Antibodies; Chromatin Proteins

References

Zhou, X., et al. Biochem. J. 418(2):277-284(2009). McCullough, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(22):7687-7691(2008). Ichioka, F., et al. FEBS J. 275(4):682-692(2008). Hanson, P.I., et al. J. Cell Biol. 180(2):389-402(2008). Shiels, A., et al. Am. J. Hum. Genet. 81(3):596-606(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 28827795
• NCBI GeneID: 128866
• NCBI Accession #: NP_789782
• NCBI GenBank Nucleotide #: NM_176812
• UniProt Accession #: Q9H444
• Molecular Weight: 25kDa
• NCBI Official Full Name: charged multivesicular body protein 4b
• NCBI Official Synonym Full Names: charged multivesicular body protein 4B
• NCBI Official Symbol: CHMP4B
• NCBI Official Synonym Symbols: SNF7; CTPP3; Shax1; CHMP4A; SNF7-2; VPS32B; CTRCT31; Vps32-2; C20orf178; dJ553F4.4
• NCBI Protein Information: charged multivesicular body protein 4b
• UniProt Protein Name: Charged multivesicular body protein 4b
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP4B
• UniProt Synonym Gene Names: C20orf178; SHAX1; CHMP4b; hSnf7-2; Vps32-2; hVps32-2
• UniProt Entry Name: CHM4B_HUMAN

NCBI Description

This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.[provided by RefSeq, Oct 2009]