Goat CHKB Polyclonal Antibody | anti-CHKB antibody
Goat anti-CHKB Antibody
Western Blot: Preliminary experiments gave an approx 38kDa band in Human Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.3kDa according to NP_005189.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI and Uniprot Product Information
NCBI Description
Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]
Uniprot Description
CHKB: Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.32; EC 2.7.1.82; Kinase, other; Lipid Metabolism - glycerophospholipid
Chromosomal Location of Human Ortholog: 22q13.33
Cellular Component: cytosol
Molecular Function: ATP binding; choline kinase activity; ethanolamine kinase activity
Biological Process: CDP-choline pathway; phosphatidylcholine biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphorylation
Disease: Muscular Dystrophy, Congenital, Megaconial Type
Research Articles on CHKB
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Product Notes
The CHKB chkb (Catalog #AAA422171) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CHKB Antibody reacts with Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CHKB can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Preliminary experiments gave an approx 38kDa band in Human Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.3kDa according to NP_005189.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the CHKB chkb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KGETLSQEEQ RKLEED. It is sometimes possible for the material contained within the vial of "CHKB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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