Rabbit Chk2 Polyclonal Antibody | anti-CHEK2 antibody

Chk2 Antibody

Cat. Num. AAA151546

• Gene Names: CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Chk2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Chk2; Polyclonal Antibody; Chk2 Antibody; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; CHK2 checkpoint homolog; Hucds1; CHK2 checkpoint homolog (S. pombe); anti-CHEK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Chk2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 543

• Applicable Applications for anti-CHEK2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Chk2 antibody can be used for detection of Chk2 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.
• Buffer: Chk2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Chk2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Chk2 expression in (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates with Chk2 antibody at 1 μg /ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 μg/mLRed: Chk2 Antibody (2391)Blue: DAPI staining)

Related Product Information for anti-CHEK2 antibody

Chk2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 6005850
• NCBI GeneID: 11200
• NCBI Accession #: NP_009125
• NCBI GenBank Nucleotide #: NM_007194.3
• UniProt Accession #: O96017; Q6QA03; Q6QA04; Q6QA05; Q6QA06; Q6QA07; Q6QA08; Q6QA10; A8K3Y9; B7ZBF3; B7ZBF4; B7ZBF5
• Molecular Weight: Predicted: 60 kDa; ; Observed: 60 kDa
• NCBI Official Full Name: serine/threonine-protein kinase Chk2 isoform a
• NCBI Official Synonym Full Names: checkpoint kinase 2
• NCBI Official Symbol: CHEK2
• NCBI Official Synonym Symbols: CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
• NCBI Protein Information: serine/threonine-protein kinase Chk2; cds1 homolog; CHK2 checkpoint homolog; checkpoint-like protein CHK2
• UniProt Protein Name: Serine/threonine-protein kinase Chk2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: CHEK2
• UniProt Synonym Gene Names: CDS1; CHK2; RAD53; Hucds1; hCds1
• UniProt Entry Name: CHK2_HUMAN

NCBI Description

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Chk2: a CAMK protein kinase of the Rad53 family. A cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Phosphorylates and inhibits CDC25C, preventing entry into mitosis, p53, leading to cell cycle arrest in G1, and BRCA1, restoring survival after DNA damage. Twelve splice variant isoforms have been described for human Chk2. LOF mutants cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype also caused by p53 mutations. Familial mutations also associated with prostate and breast cancer, and mutations also seen in a variety of sporadic cancers and cell lines.

Protein type: Protein kinase, CAMK; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; RAD53 family

Chromosomal Location of Human Ortholog: 22q12.1

Cellular Component: nucleoplasm; Golgi apparatus; PML body; chromosome, telomeric region

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; ubiquitin protein ligase binding; metal ion binding; protein kinase binding; ATP binding

Biological Process: protein stabilization; DNA damage induced protein phosphorylation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; DNA damage response, signal transduction; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; cell division; cellular protein catabolic process; double-strand break repair; DNA damage checkpoint; response to gamma radiation; regulation of protein catabolic process; G2/M transition of mitotic cell cycle; response to DNA damage stimulus

Disease: Li-fraumeni Syndrome 2; Prostate Cancer; Breast Cancer; Osteogenic Sarcoma