Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK1 antibody (Ser317))
Rabbit CHK1 Polyclonal Antibody | anti-CHK1 antibody
CHK1 Antibody (Ser317)
Gene Names
CHEK1; CHK1
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
CHK1; Polyclonal Antibody; CHK1 Antibody (Ser317); Rabbit Polyclonal CHK1 Antibody (Ser317); Polyclonal CHK1 antibody; Anti-CHK1 antibody; Checkpoint S. pombe homolog of 1 antibody; CHEK1 antibody; CHK1 antibody; CHK1 checkpoint homolog (S. pombe) antibody; Serine/threonine-protein kinase Chk1 antibody; anti-CHK1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
CHK1 antibody (Ser317) detects endogenous levels of CHK1 only when phosphorylated at serine 317
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
476
Applicable Applications for anti-CHK1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:4000
IHC: 1:50-1:100
ELISA: 1:4000
Immunogen
CHK1 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human CHK1 around the phosphorylation site of serine 317 (S-S-SP-Q-P) as the immunogen.
Cross-Reactivity
Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK1 antibody (Ser317))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK1 antibody (Ser317))
Related Product Information for anti-CHK1 antibody
CHK1 is a serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA.
Product Categories/Family for anti-CHK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Chk1
NCBI Official Synonym Full Names
checkpoint kinase 1
NCBI Official Symbol
CHEK1
NCBI Official Synonym Symbols
CHK1
NCBI Protein Information
serine/threonine-protein kinase Chk1
Protein Family
NCBI Description
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
Research Articles on CHK1
1. Cellular conditions, especially cell density, influenced the target engagement of V158411 for Chk1.
Similar Products
Product Notes
The CHK1 (Catalog #AAA5313772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CHK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 ELISA: 1:4000. Researchers should empirically determine the suitability of the CHK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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