Rabbit Chk1 Polyclonal Antibody | anti-CHEK1 antibody

Chk1, phosphorylated (Ser345) (CHEK1, Cell Cycle Checkpoint Kinase)

Cat. Num. AAA620911

• Gene Names: CHEK1; CHK1
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: Chk1; Polyclonal Antibody; phosphorylated (Ser345) (CHEK1; Cell Cycle Checkpoint Kinase); Anti -Chk1; anti-CHEK1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human Chk1 at ~56kD only when activated by phosphorylation at serine 345. Does not recognize Chk1 phosphorylated at other sites. Species Crossreactivity: monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-CHEK1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser345 of human Chk1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from UV or MMS treated COS cells, using MBS620911.)

Western Blot (WB)

(Western Blot analysis of extracts from C6 and HeLa cells treated with UV, MMS or HU as indicated using MBS620911.)

Related Product Information for anti-CHEK1 antibody

Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression. Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress. Phosphorylation at Ser 345 serves to localize Chk1 to the nucleus following checkpoint acitvation while recently phosphorylation at Ser 317 along with site-specific phosphorylation of PTEN was shown to allow for reentry into the cell cycle following stalled DNA replication. Chk1 exerts it checkpoint mechanism on the cell cycle in part by regulating the cdc25 family of phosphatases. Chk1 phosphorylation of cdc25A targets it for proteolysis and inhibits it's activity though 14-3-3 binding.. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis. Also, centrosomal Chk1 has been shown to phosphorylate cdc25B inhibiting its activation of CDK1-cyclin B1 and thus mitotic spindle formation and chromatin condensation. Furthermore, Chk1 plays a role in spindle checkpoint function through regulation of Aurora B and BubR1. Chk1 has emerged as a drug target for cancer therapy as its inhibition leads to cell death in many cancer cell lines.

Product Categories/Family for anti-CHEK1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 2367669
• NCBI GeneID: 1111
• UniProt Accession #: O14757; A8K934; Q6W8P8
• Molecular Weight: 54,434 Da
• NCBI Official Full Name: Chk1
• NCBI Official Synonym Full Names: CHK1 checkpoint homolog (S. pombe)
• NCBI Official Symbol: CHEK1
• NCBI Official Synonym Symbols: CHK1
• NCBI Protein Information: serine/threonine-protein kinase Chk1; OTTHUMP00000231300; OTTHUMP00000231301; OTTHUMP00000231306; OTTHUMP00000231307; OTTHUMP00000231309; Checkpoint, S. pombe, homolog of, 1
• UniProt Protein Name: Serine/threonine-protein kinase Chk1
• UniProt Gene Name: CHEK1
• UniProt Synonym Gene Names: CHK1
• UniProt Entry Name: CHK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.1; Tumor suppressor; CAMK group; CAMKL family; CHK1 subfamily

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: nucleoplasm; centrosome; extracellular space; chromosome, telomeric region; condensed nuclear chromosome; intracellular membrane-bound organelle; replication fork; nucleus; chromatin; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: DNA damage induced protein phosphorylation; chromatin-mediated maintenance of transcription; regulation of mitotic centrosome separation; DNA damage checkpoint; negative regulation of mitosis; peptidyl-threonine phosphorylation; G2/M transition of mitotic cell cycle; DNA repair; DNA replication; G2/M transition DNA damage checkpoint; response to DNA damage stimulus; regulation of cell proliferation