Western Blot (WB)
((1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CHIT1 Polyclonal Antibody | anti-CHIT1 antibody
Goat anti-CHIT1 (aa409-423) Antibody
Western Blot: Approx 48kDa band observed in lysates of cell line Daudi (calculated MW of 51.7kDa according to NP_003456.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; EC 3.2.1.14; Secreted; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Hydrolase
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: extracellular region; extracellular space; lysosome
Molecular Function: chitin binding; chitinase activity; endochitinase activity; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: carbohydrate metabolic process; chitin catabolic process; immune response; polysaccharide catabolic process; response to bacterium
Disease: Chitotriosidase Deficiency
Research Articles on CHIT1
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Product Notes
The CHIT1 chit1 (Catalog #AAA422744) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CHIT1 (aa409-423) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHIT1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 48kDa band observed in lysates of cell line Daudi (calculated MW of 51.7kDa according to NP_003456.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the CHIT1 chit1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EHGPSPGQDT FCQGK. It is sometimes possible for the material contained within the vial of "CHIT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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