Rabbit anti-Human CHIP Polyclonal Antibody | anti-CHIP antibody

CHIP, CT (E3 Ubiquitin-protein Ligase CHIP, Carboxy Terminus of HSP70-interacting Protein, CLL-associated Antigen KW-8, STIP1 Homology and U-Box Containing Protein 1, Antigen NY-CO-7, STUB1, PP1131) (APC)

Cat. Num. AAA6468705

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CHIP; Polyclonal Antibody; CT (E3 Ubiquitin-protein Ligase CHIP; Carboxy Terminus of HSP70-interacting Protein; CLL-associated Antigen KW-8; STIP1 Homology and U-Box Containing Protein 1; Antigen NY-CO-7; STUB1; PP1131) (APC); anti-CHIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHIP.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CHIP antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide at the C-term of human CHIP (STUB1) (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CHIP (STUB1) Antibody (C-term) in Ramos cell line lysates (35ug/lane). CHIP (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CHIP (STUB1) antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CHIP antibody

Antibodies; Ubiquitin Ligases

References

1. Peng, H.M., et al. J. Biol. Chem. 279(51), 52970-52977 (2004). 2. Alberti, S., et al. Mol. Biol. Cell 15(9), 4003-4010 (2004). 3. Beausoleil, S.A., et al. PNAS 101(33), 12130-12135 (2004). 4. He, B., et al. J. Biol. Chem. 279(29), 30643-30653 (2004). 5. Petrucelli, L., et al. Hum. Mol. Genet. 13(7), 703-714 (2004). 6.Shimura, H., et al. J. Biol. Chem. 279(6), 4869-4876 (2004). 7.Li, L., et al. Mol. Cell. Biol. 24(2), 856-864 (2004). 8.Zhou, P., et al. J. Biol. Chem. 278(16), 13829-13837 (2003). 9.Imai, Y., et al. Mol. Cell 10(1), 55-67 (2002). 10.Ballinger, C.A., et al. Mol. Cell. Biol. 19(6), 4535-4545 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 74760687
• UniProt Accession #: Q969U2
• Molecular Weight: 34.856
• Protein Family: Chitoporin