The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide
recombinant proteins, antibodies and ELISA kits to most target proteins.
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '30849'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
1.62 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '30849' and pd.language_id = 1
Query
Database
1.23 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '30849'
Query
Database
7.51 ms
UPDATE `products_description` SET `products_viewed` = products_viewed + 1
WHERE `products_id` = 30849
Database (5 total Queries, 5 of them unique across 2 Connections)
Time
Query String
1.97 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '30849'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '30849' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '30849'
Application Data||AAA30849_APP6.png!!Application Data||AAA30849_APP5.png!!Application Data||AAA30849_APP4.png!!Application Data||AAA30849_APP3.png!!Application Data||AAA30849_APP2.png!!SDS-PAGE||Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blott||AAA30849_SDS_PAGE.png
Application Data||AAA30849_APP6.png!!Application Data||AAA30849_APP5.png!!Application Data||AAA30849_APP4.png!!Application Data||AAA30849_APP3.png!!Application Data||AAA30849_APP2.png!!SDS-PAGE||Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blott||AAA30849_SDS_PAGE.png
Application Data||AAA30849_APP6.png!!Application Data||AAA30849_APP5.png!!Application Data||AAA30849_APP4.png!!Application Data||AAA30849_APP3.png!!Application Data||AAA30849_APP2.png!!SDS-PAGE||Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blott||AAA30849_SDS_PAGE.png
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$value[0]['_source']['products_description']
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress and the regulation of cellular redox conditions. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of N...
$value[1]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of NFE2R2. No significant cross-reactivity or interference between NFE2R2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFE2R2 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1365) "Intended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designe...
$value[1]['_source']['products_description']
Intended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NFE2R2. This ELISA kit for research use only!<br><br>Principle of the Assay: NFE2R2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NFE2R2 antibody and an NFE2R2-HRP conjugate. The assay sample and buffer are incubated together with NFE2R2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFE2R2 concentration since NFE2R2 from samples and NFE2R2-HRP conjugate compete for the anti-NFE2R2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFE2R2 from the sample, fewer sites are left to bind NFE2R2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFE2R2 concentration in each sample is interpolated from this standard curve.
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⇄⧉products_related_diseases => string (230) "Inflammation||527!!Disease Models, Animal||297!!Nervous System Diseases||295...
⇄⧉search_terms => string (721) "aaa17206 rat this assay has high sensitivity and excellent specificity for d...
$value[1]['_source']['search_terms']
aaa17206 rat this assay has high sensitivity and excellent specificity for detection of nfe2r2 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17206_sc elisa kit nuclear factor erythroid 2 related nrf2 isoform like nfe2l2 hebp1 nfe2 nf e2 derived 65,356 da nf2l2_human 224028257 np_001138884.1 q16236 nm_001145412.2 q53rw6 q59hh2 q96f71 b2rbu2 b4e338 e9pgj7 600492 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml erythroid2 competitive0.1
Application Data||AAA30849_APP6.png!!Application Data||AAA30849_APP5.png!!Application Data||AAA30849_APP4.png!!Application Data||AAA30849_APP3.png!!Application Data||AAA30849_APP2.png!!SDS-PAGE||Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blott||AAA30849_SDS_PAGE.png
IHC (Immunohistochemistry)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.||AAA28853_IHC7.jpg!!IHC (Immunohistchemistry)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.||AAA28853_IHC6.jpg!!IHC (Immunohistochemistry)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.||AAA28853_IHC5.jpg!!IHC (Immunohistochemistry-Paraffin)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.||AAA28853_IHC4.jpg!!IHC (Immunohistochemistry-Paraffin)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.||AAA28853_IHC3.jpg!!IHC (Immunohistochemistry-Paraffin)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.||AAA28853_IHC2.jpg!!WB (Western Blot)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.||AAA28853_WB.jpg
⇄⧉products_description => string (431) "Transcription activator that binds to antioxidant response (ARE) elements in...
$value[3]['_source']['products_description']
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress and the regulation of cellular redox conditions. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of N...
$value[4]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of NFE2R2. No significant cross-reactivity or interference between NFE2R2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFE2R2 and all the analogues, therefore, cross reaction may still exist in some cases.
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive or Sandwich!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||0.1 ng/ml
⇄⧉products_description => string (1415) "Principle of the Assay: NFE2R2 ELISA kit applies the competitive enzyme immu...
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Principle of the Assay: NFE2R2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NFE2R2 antibody and an NFE2R2-HRP conjugate. The assay sample and buffer are incubated together with NFE2R2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFE2R2 concentration since NFE2R2 from samples and NFE2R2-HRP conjugate compete for the anti-NFE2R2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFE2R2 from the sample, fewer sites are left to bind NFE2R2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFE2R2 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NFE2R2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄products_references => string (3) "N/A"
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⇄⧉products_related_diseases => string (230) "Inflammation||527!!Disease Models, Animal||297!!Nervous System Diseases||295...
⇄⧉search_terms => string (729) "aaa17077 mouse this assay has high sensitivity and excellent specificity for...
$value[4]['_source']['search_terms']
aaa17077 mouse this assay has high sensitivity and excellent specificity for detection of nfe2r2 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17077_sc elisa kit nuclear factor erythroid 2 related nrf2 isoform like nfe2l2 hebp1 nfe2 nf e2 derived 65,356 da nf2l2_human 224028257 np_001138884.1 q16236 nm_001145412.2 q53rw6 q59hh2 q96f71 b2rbu2 b4e338 e9pgj7 600492 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive sandwich 0.1 ng ml erythroid2 sandwich0.1
⇄⧉testing_protocols => string (1231) "IF (Immunofluorescence)||Immunofluorescence analysis of HepG2 using NRF2 ant...
$value[5]['_source']['testing_protocols']
IF (Immunofluorescence)||Immunofluorescence analysis of HepG2 using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.||AAA28410_IF6.jpg!!IF (Immunofluorescence)||Immunofluorescence analysis of HeLa using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.||AAA28410_IF5.jpg!!IF (Immunofluorescence)||Immunofluorescence analysis of PC-12 using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.||AAA28410_IF4.jpg!!IF (Immunofluorescence)||Immunofluorescence analysis of NIH-3T3 using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.||AAA28410_IF3.jpg!!IHC (Immunohistochemistry)||Immunohistochemistry of paraffin-embedded human placenta using NRF2 Rabbit pAb at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.||AAA28410_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemistry of paraffin-embedded human esophageal cancer using NRF2 Rabbit pAb at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.||AAA28410_IHC.jpg
⇄⧉etc_term1 => string (115) "Positive Samples||C6!!Immunogen||Recombinant protein of human NRF2.!!Cellula...
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Positive Samples||C6!!Immunogen||Recombinant protein of human NRF2.!!Cellular Location||Cytoplasm, Nucleus, cytosol
⇄⧉products_description => string (464) "This gene encodes a transcription factor which is a member of a small family...
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This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Application Data||Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300. Not yet tested in other applications.||AAA28894_APP6.jpg!!Application Data||Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300. Not yet tested in other applications.||AAA28894_APP5.jpg!!Application Data||Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300. Not yet tested in other applications.||AAA28894_APP4.jpg!!Application Data||Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300. Not yet tested in other applications.||AAA28894_APP3.jpg!!Application Data||Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300. Not yet tested in other applications.||AAA28894_APP2.jpg!!WB (Western Blot)||Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300. Not yet tested in other applications.||AAA28894_WB.jpg
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Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress and the regulation of cellular redox conditions. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
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This assay has high sensitivity and excellent specificity for detection of NFE2R2. No significant cross-reactivity or interference between NFE2R2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFE2R2 and all the analogues, therefore, cross reaction may still exist in some cases.
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1417) "Principle of the Assay: NFE2R2 ELISA kit applies the competitive enzyme immu...
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Principle of the Assay: NFE2R2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NFE2R2 antibody and an NFE2R2-HRP conjugate. The assay sample and buffer are incubated together with NFE2R2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFE2R2 concentration since NFE2R2 from samples and NFE2R2-HRP conjugate compete for the anti-NFE2R2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFE2R2 from the sample, fewer sites are left to bind NFE2R2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFE2R2 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken NFE2R2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄products_references => string (3) "N/A"
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⇄⧉products_related_diseases => string (230) "Inflammation||527!!Disease Models, Animal||297!!Nervous System Diseases||295...
⇄⧉search_terms => string (480) "aaa17229 chicken typical testing data standard curve for reference only aaa1...
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aaa17229 chicken typical testing data standard curve for reference only aaa17229_sc elisa kit nuclear factor erythroid 2 related nrf2 isoform like nfe2l2 hebp1 nfe2 nf e2 derived 65,356 da nf2l2_human 224028257 np_001138884.1 q16236 nm_001145412.2 q53rw6 q59hh2 q96f71 b2rbu2 b4e338 e9pgj7 600492 signal transduction samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type quantitative competitive sensitivity 1.0 ng ml erythroid2 sensitivity1.0
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids!!Detection Range||0.2-60ng/ml!!Sensitivity||0.108ng/ml
⇄⧉etc_term2 => string (406) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
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Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV< 10%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV< 12%
⇄⧉products_description => string (910) "Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (EL...
$value[8]['_source']['products_description']
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse NRF2 antibody. NRF2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NRF2 Antibody is added and binds to NRF2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NRF2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse NRF2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.<br><br>Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Nuclear factor erythroid 2-related factor 2 (also known as NRF2) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
⇄⧉search_terms => string (651) "aaa19022 mouse typical testing data standard curve for reference only aaa190...
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aaa19022 mouse typical testing data standard curve for reference only aaa19022_sc elisa kit nuclear factor erythroid 2 related nrf2 isoform 6 like nfe2l2 hebp1 imddhh 66,076 da derived nf e2 nfe2 926657654 np_001300833.1 q16236 nm_001313904.1 q53rw6 q59hh2 q96f71 b2rbu2 b4e338 e9pgj7 samples serum plasma cell culture supernates ascites tissue homogenates or other biological fluids assay type quantitative sandwich detection range 0.2 60ng ml sensitivity 0.108ng intra precision within an three of known concentration were tested on one plate to assess cv<8 inter between assays in separate cv = sd mean x 100 cv<10 erythroid2 isoform6 range0.2 x100
⇄⧉products_description => string (684) "Intended Uses: This NRF2 ELISA kit is intended for laboratory research use o...
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Intended Uses: This NRF2 ELISA kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of NRF2 in the sample, this NRF2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed simultaneously with the samples and allow the operator to produce a standard curve of Optical Density versus NRF2 concentration. The concentration of NRF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄products_references => string (3) "N/A"
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⇄products_related_diseases => string (3) "N/A"
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⇄ncbi_full_name => string (3) "N/A"
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⇄ncbi_full_name_syn => string (3) "N/A"
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⇄ncbi_symbol => string (3) "N/A"
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⇄ncbi_symbol_syn => string (3) "N/A"
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⇄ncbi_protein_info => string (3) "N/A"
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⇄ncbi_chrom_loc => string (3) "N/A"
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⇄ncbi_gene_id => string (3) "N/A"
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⇄ncbi_mol_weight => string (3) "N/A"
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⇄ncbi_pathways => string (3) "N/A"
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⇄sp_protein_name => string (3) "N/A"
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⇄sp_protein_name_syn => string (3) "N/A"
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⇄sp_gene_name => string (3) "N/A"
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⇄sp_gene_name_syn => string (3) "N/A"
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⇄sp_entry_name => string (3) "N/A"
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⇄sp_mim => string (3) "N/A"
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⇄sp_interactions => string (3) "N/A"
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⇄products_url => string (3) "N/A"
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⇄products_viewed => string (1) "0"
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⇄⧉search_terms => string (130) "aaa23628 human typical testing data standard curve for reference only aaa236...
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aaa23628 human typical testing data standard curve for reference only aaa23628_sc elisa kit nuclear factor e2 related factor2 nrf2
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of N...
$value[10]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of NFE2R2. No significant cross-reactivity or interference between NFE2R2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFE2R2 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
$value[10]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1415) "Principle of the Assay: NFE2R2 ELISA kit applies the competitive enzyme immu...
$value[10]['_source']['products_description']
Principle of the Assay: NFE2R2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NFE2R2 antibody and an NFE2R2-HRP conjugate. The assay sample and buffer are incubated together with NFE2R2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFE2R2 concentration since NFE2R2 from samples and NFE2R2-HRP conjugate compete for the anti-NFE2R2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFE2R2 from the sample, fewer sites are left to bind NFE2R2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFE2R2 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NFE2R2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄products_references => string (3) "N/A"
$value[10]['_source']['products_references']
⇄⧉products_related_diseases => string (230) "Inflammation||527!!Disease Models, Animal||297!!Nervous System Diseases||295...
⇄⧉search_terms => string (723) "aaa17087 human this assay has high sensitivity and excellent specificity for...
$value[10]['_source']['search_terms']
aaa17087 human this assay has high sensitivity and excellent specificity for detection of nfe2r2 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17087_sc elisa kit nuclear factor erythroid 2 related nrf2 isoform like nfe2l2 hebp1 nfe2 nf e2 derived 65,356 da nf2l2_human 224028257 np_001138884.1 q16236 nm_001145412.2 q53rw6 q59hh2 q96f71 b2rbu2 b4e338 e9pgj7 600492 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml erythroid2 competitive0.1
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
$value[11]['_source']['etc_term2']
Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
⇄⧉products_description => string (860) "Intended Uses: This sandwich kit is for the accurate quantitative detection ...
$value[11]['_source']['products_description']
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Leptin Receptor (also known as LEPR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.<br><br>Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human LEPR antibody. LEPR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human LEPR Antibody is added and binds to LEPR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LEPR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human LEPR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
⇄products_references => string (3) "N/A"
$value[11]['_source']['products_references']
⇄⧉products_related_diseases => string (225) "Peripheral Nervous System Diseases||396!!Drug Toxicity||335!!Obesity||302!!O...
Peripheral Nervous System Diseases||396!!Drug Toxicity||335!!Obesity||302!!Overweight||213!!Diabetes Mellitus||184!!Inflammation||178!!Neoplasms||146!!Necrosis||102!!Diabetes Mellitus, Type 2||100!!Cardiovascular Diseases||97
⇄⧉search_terms => string (547) "aaa11140 human typical testing data standard curve for reference only aaa111...
$value[11]['_source']['search_terms']
aaa11140 human typical testing data standard curve for reference only aaa11140_sc elisa kit leptin receptor lepr obr ob r cd295 lep leprd 102,490 da hub219 cd_antigen db 3236286 aac23650.1 p48357 q13592 q13593 q13594 q92919 q92920 q92921 samples serum plasma cell culture supernates lysates tissue homogenates assay type quantitative sandwich detection range 2ng ml 600ng sensitivity 0.9ng intra precision within an three of known concentration were tested on one plate to assess cv<8 inter between assays in separate cv = sd mean x 100 cv<10 x100
⇄⧉specificity => string (379) "This assay has high sensitivity and excellent specificity for detection of s...
$value[12]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of sLEPR. No significant cross-reactivity or interference between sLEPR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sLEPR and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[12]['_source']['purity']
⇄form => string (3) "N/A"
$value[12]['_source']['form']
⇄concentration => string (3) "N/A"
$value[12]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1396) "Intended Uses: This sLEPR ELISA kit is a 1.5 hour solid-phase ELISA designed...
$value[12]['_source']['products_description']
Intended Uses: This sLEPR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse sLEPR. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: sLEPR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sLEPR antibody and an sLEPR-HRP conjugate. The assay sample and buffer are incubated together with sLEPR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sLEPR concentration since sLEPR from samples and sLEPR-HRP conjugate compete for the anti-sLEPR antibody binding site. Since the number of sites is limited, as more sites are occupied by sLEPR from the sample, fewer sites are left to bind sLEPR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sLEPR concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (556) "aaa16027 mouse this assay has high sensitivity and excellent specificity for...
$value[12]['_source']['search_terms']
aaa16027 mouse this assay has high sensitivity and excellent specificity for detection of slepr no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16027_sc elisa kit soluble leptin receptor slr signal transduction samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml competitive0.1
⇄⧉specificity => string (379) "This assay has high sensitivity and excellent specificity for detection of S...
$value[13]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of SLEPR. No significant cross-reactivity or interference between SLEPR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLEPR and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[13]['_source']['purity']
⇄form => string (3) "N/A"
$value[13]['_source']['form']
⇄concentration => string (3) "N/A"
$value[13]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1400) "Principle of the Assay: SLEPR ELISA kit applies the competitive enzyme immun...
$value[13]['_source']['products_description']
Principle of the Assay: SLEPR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SLEPR antibody and an SLEPR-HRP conjugate. The assay sample and buffer are incubated together with SLEPR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLEPR concentration since SLEPR from samples and SLEPR-HRP conjugate compete for the anti-SLEPR antibody binding site. Since the number of sites is limited, as more sites are occupied by SLEPR from the sample, fewer sites are left to bind SLEPR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLEPR concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This SLEPR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SLEPR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (554) "aaa16328 rat this assay has high sensitivity and excellent specificity for d...
$value[13]['_source']['search_terms']
aaa16328 rat this assay has high sensitivity and excellent specificity for detection of slepr no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16328_sc elisa kit soluble leptin receptor slr signal transduction samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml competitive0.1
⇄⧉specificity => string (175) "This assay has high sensitivity and excellent specificity for detection of r...
$value[14]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of rat LEP. No significant cross-reactivity or interference between rat LEP and analogues was observed.
⇄purity => string (3) "N/A"
$value[14]['_source']['purity']
⇄form => string (3) "N/A"
$value[14]['_source']['form']
⇄concentration => string (3) "N/A"
$value[14]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[14]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[14]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (727) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[14]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEP bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (596) "aaa15189 rat this assay has high sensitivity and excellent specificity for d...
$value[14]['_source']['search_terms']
aaa15189 rat this assay has high sensitivity and excellent specificity for detection of human lep no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15189_td elisa kit leptin flj94114 ob obs murine obesity homolog mouse obese factor 18,866 da lep_rat 6981148 np_037208.1 p50596 nm_013076.3 samples serum plasma tissue homogenates type quantitative sandwich range 0.156 ng ml 10 0.060 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml10
⇄⧉specificity => string (179) "This assay has high sensitivity and excellent specificity for detection of h...
$value[15]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of horse LEP. No significant cross-reactivity or interference between horse LEP and analogues was observed.
⇄purity => string (3) "N/A"
$value[15]['_source']['purity']
⇄form => string (3) "N/A"
$value[15]['_source']['form']
⇄concentration => string (3) "N/A"
$value[15]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[15]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[15]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (615) "Principle of the Assay: This assay employs the competitive inhibition enzyme...
$value[15]['_source']['products_description']
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LEP. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LEP. The competitive inhibition reaction is launched between with pre-coated LEP and LEP in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (599) "aaa18302 horse this assay has high sensitivity and excellent specificity for...
$value[15]['_source']['search_terms']
aaa18302 horse this assay has high sensitivity and excellent specificity for detection of lep no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18302_td elisa kit leptin flj94114 ob obs murine obesity homolog mouse obese factor 15,935 da lep_horse 57015328 q9tu09.2 q9tu09 q6s9b2 q9xs85 samples serum plasma tissue homogenates type quantitative competitive range 3.12 pg ml 200 < 0.78 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml200
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
⇄app_notes => string (19) "FC/FACS: 1:50-1:100"
$value[16]['_source']['app_notes']
⇄⧉testing_protocols => string (1406) "FCM (Flow Cytometry)||Flow cytometric analysis of K562 cells with Leptin Rec...
$value[16]['_source']['testing_protocols']
FCM (Flow Cytometry)||Flow cytometric analysis of K562 cells with Leptin Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).||AAA30347_FCM7.jpg!!ICC (Immunocytochemistry)||ICC staining Leptin Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30347_ICC6.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.||AAA30347_IHC5.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.||AAA30347_IHC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.||AAA30347_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.||AAA30347_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.||AAA30347_IHC.jpg
⇄⧉products_name_syn => string (478) "CD 295 antibody; CD295 antibody; CD295 antigen antibody; Db antibody; Fa ant...
$value[16]['_source']['products_name_syn']
CD 295 antibody; CD295 antibody; CD295 antigen antibody; Db antibody; Fa antibody; HuB219 antibody; LEP R antibody; LEP-R antibody; LEPR antibody; LEPR_HUMAN antibody; LEPRD antibody; Leptin receptor antibody; Leptin receptor fatty antibody; Leptin receptor gene related protein antibody; Leptin receptor precursor antibody; Leptin receptor precursor antibody; OB R gene related protein antibody; OB receptor antibody; OB-R antibody; OB-RGRP antibody; obl antibody; Obr antibody
⇄products_gene_name => string (3) "N/A"
$value[16]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[16]['_source']['products_gene_name_syn']
⇄⧉products_description => string (289) "Receptor for obesity factor (leptin). On ligand binding, mediates signaling ...
$value[16]['_source']['products_description']
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
⇄products_references => string (3) "N/A"
$value[16]['_source']['products_references']
⇄⧉products_related_diseases => string (224) "Peripheral Nervous System Diseases||396!!Drug Toxicity||335!!Obesity||299!!O...
Peripheral Nervous System Diseases||396!!Drug Toxicity||335!!Obesity||299!!Overweight||211!!Diabetes Mellitus||182!!Inflammation||177!!Neoplasms||146!!Necrosis||101!!Diabetes Mellitus, Type 2||98!!Cardiovascular Diseases||96
⇄products_categories => string (16) "Total protein Ab"
⇄⧉search_terms => string (1062) "aaa30347 rabbit human mouse rat monoclonal ja73 01 proa affinity purified 1*...
$value[16]['_source']['search_terms']
aaa30347 rabbit human mouse rat monoclonal ja73 01 proa affinity purified 1*tbs ph7.4 1 bsa 40 glycerol preservative 0.05 sodium azide western blot wb immunocytochemistry icc immunohistochemistry ihc flow cytometry fc facs 1:50 1:100 immunohistochemical analysis of paraffin embedded heart tissue using anti leptin receptor antibody counter stained with hematoxylin aaa30347_ihc liver cancer aaa30347_ihc2 small intestine aaa30347_ihc3 prostate aaa30347_ihc4 kidney aaa30347_ihc5 staining in mcf 7 cells green the nuclear stain is dapi blue were fixed paraformaldehyde permeabilised 0.25 triton x100 pbs aaa30347_icc6 cytometric k562 at 100 dilution red compared an unlabelled control without incubation primary black aaa30347_fc7 cd 295 cd295 antigen db fa hub219 lep r lepr lepr_human leprd fatty gene related protein precursor ob rgrp obl obr isoform 3 132,494 da 51093379 np_001003679.1 p48357 nm_001003679.3 q13592 q13593 q13594 q92919 q92920 q92921 601007 total ab type recombinant immunogen conjugation unconjugated ja7301 ph7.41 bsa40 mcf7 at100 isoform3
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of L...
$value[17]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of LEP. No significant cross-reactivity or interference between LEP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LEP and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[17]['_source']['purity']
⇄form => string (3) "N/A"
$value[17]['_source']['form']
⇄concentration => string (3) "N/A"
$value[17]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1377) "Principle of the Assay: LEP ELISA kit applies the competitive enzyme immunoa...
$value[17]['_source']['products_description']
Principle of the Assay: LEP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LEP antibody and an LEP-HRP conjugate. The assay sample and buffer are incubated together with LEP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LEP concentration since LEP from samples and LEP-HRP conjugate compete for the anti-LEP antibody binding site. Since the number of sites is limited, as more sites are occupied by LEP from the sample, fewer sites are left to bind LEP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LEP concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This LEP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine LEP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (606) "aaa17041 bovine this assay has high sensitivity and excellent specificity fo...
$value[17]['_source']['search_terms']
aaa17041 bovine this assay has high sensitivity and excellent specificity for detection of lep no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17041_sc elisa kit leptin leptin< ob xlep obesity homolog 148233267 np_001089183.1 nm_001095714.1 signal transduction samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml competitive0.1
⇄⧉products_description => string (1104) "Intended Uses: This immunoassay kit allows for the in vitro quantitative det...
$value[18]['_source']['products_description']
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.<br><br>Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄⧉search_terms => string (419) "aaa23205 bovine recombinant and natural leptin typical testing data standard...
$value[18]['_source']['search_terms']
aaa23205 bovine recombinant and natural leptin typical testing data standard curve for reference only aaa23205_sc elisa kit obesity factor lep ob obs 18,716 da lep_bovin 87196505 np_776353.2 p50595 nm_173928.2 o97918 q5eae4 q95133 q9tsz9 samples serum plasma tissue homogenates cell culture supernates or other biological fluids detection range 0.156 10 ng ml sensitivity < 0.069 intra assay precision <=4.6 inter <=7.9
⇄⧉specificity => string (364) "The OmniKine Human Heregulinbeta-1 ELISA is capable of recognizing both reco...
$value[19]['_source']['specificity']
The OmniKine Human Heregulinbeta-1 ELISA is capable of recognizing both recombinant and naturally produced Human Heregulinbeta-1 proteins. The antigens listed below were tested at 50ng/ml the following antigens did not exhibit significant cross reactivity Human EGF, EGF Receptor, EG-VEGF, Epigen, Epiregulin, HB-EGF, TGF-alpha, VEGF Murine EGF, VEGF Rat EGF, VEGF
⇄purity => string (3) "N/A"
$value[19]['_source']['purity']
⇄form => string (23) "12 x 8-Well Microstrips"
$value[19]['_source']['form']
⇄concentration => string (3) "N/A"
$value[19]['_source']['concentration']
⇄⧉storage_stability => string (91) "Store entire kit at 4 degree C until use. Kit expiration is 3 months from da...
$value[19]['_source']['storage_stability']
Store entire kit at 4 degree C until use. Kit expiration is 3 months from date of shipment.
⇄⧉products_description => string (2221) "Principle of the Assay: The OmniKine Human Heregulinbeta-1 ELISA Kit contain...
$value[19]['_source']['products_description']
Principle of the Assay: The OmniKine Human Heregulinbeta-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Heregulinbeta-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Heregulinbeta-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human Heregulinbeta-1 is an isoform of the gene product NRG1 through alternative splicing. A great variety of isoforms are known to be produced from NRG1 and each isoform is tissue specific and have structural differences. HRG isoforms all contain immunoglobulin and epidermal growth factor like domains. NRG1 isoforms are known to play roles in growth and differentiation of many different cell types.
⇄⧉search_terms => string (605) "aaa30734 human 12 x 8 well microstrips the omnikine heregulinbeta 1 elisa is...
$value[19]['_source']['search_terms']
aaa30734 human 12 x 8 well microstrips the omnikine heregulinbeta 1 elisa is capable of recognizing both recombinant and naturally produced proteins antigens listed below were tested at 50ng ml following did not exhibit significant cross reactivity egf receptor eg vegf epigen epiregulin hb tgf alpha murine rat typical testing data standard curve for reference only aaa30734_sc kit leptin obese protein obesity factor ob obs lep lepd 18,641 da 4557715 np_000221.1 p41159 nm_000230.2 o15158 q56a88 u18915 mrna assay type quantitative sandwich sensitivity 32 2000 pg human12 x8 heregulinbeta1 sensitivity32
