Goat CHD1 Polyclonal Antibody | anti-CHD1 antibody
Goat anti-CHD1 (internal) Antibody
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CHD1; Polyclonal Antibody; Goat anti-CHD1 (internal) Antibody; Goat Anti-CHD1 (C Terminus) Antibody; chromodomain helicase DNA binding protein 1; DKFZp686E2337; CHD1 antibody; chromodomain helicase DNA binding protein 1 antibody; DKFZp686E2337 antibody; CHD1 (internal); anti-CHD1 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
NGKDHRDWDHYKQD
Sequence Length
1710
Applicable Applications for anti-CHD1 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Preliminary experiments gave bands at approx 110kDa and 30kDa in lysates of cell line HeLa after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 197kDa according to NP_001261.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Western Blot: Preliminary experiments gave bands at approx 110kDa and 30kDa in lysates of cell line HeLa after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 197kDa according to NP_001261.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunogen
Peptide with sequence C-NGKDHRDWDHYKQD, from the internal region of the protein sequence according to NP_001261.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
196,590 Da
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 1
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 1
NCBI Official Symbol
CHD1
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 1
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 1
Protein Family
UniProt Gene Name
CHD1
UniProt Synonym Gene Names
CHD-1
UniProt Entry Name
CHD1_HUMAN
NCBI Description
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. [provided by RefSeq, Jul 2008]
Uniprot Description
CHD-1: a sequence-selective DNA-binding protein. Contains two chromo (chromatin organization modifier) domains and one SNF2-related helicase and ATPase domains. May play an important role in gene regulation.
Protein type: Helicase; EC 3.6.4.12
Chromosomal Location of Human Ortholog: 5q15-q21
Cellular Component: cytoplasm; nucleus
Molecular Function: ATP binding; ATP-dependent DNA helicase activity; DNA binding; methylated histone residue binding; protein binding
Biological Process: chromatin remodeling; DNA duplex unwinding; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
Research Articles on CHD1
1. We have identified CHD1 as the RUNX1 fusion partner in acute myeloid leukemia with t(5;21)(q21;q22).
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Product Notes
The CHD1 chd1 (Catalog #AAA421541) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CHD1 (internal) Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CHD1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Preliminary experiments gave bands at approx 110kDa and 30kDa in lysates of cell line HeLa after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 197kDa according to NP_001261.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Researchers should empirically determine the suitability of the CHD1 chd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NGKDHRDWDH YKQD. It is sometimes possible for the material contained within the vial of "CHD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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