Western Blot (WB)
(CL7812AP (0.01ug/ml) staining of human skin (A) and MCF7 cell line (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat anti-Human CGRP Polyclonal Antibody | anti-CGRP antibody
Anti-Human CGRP, Purified, (Polyclonal)(Goat IgG)
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Purified. Affinity Chromatography
Synonyms
CGRP; Polyclonal Antibody; Anti-Human CGRP; Purified; (Polyclonal)(Goat IgG); Calcitonin gene related peptide; anti-CGRP antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
Goat IgG
Specificity
This antibody is specific for human Calcitonin gene related peptide(CGRP)
Purity/Purification
Purified. Affinity Chromatography
Form/Format
Purified IgG (1mg/ml)buffered in PBS and 0.02% NaN3. (Purified from serum via Affinity Chromatography).
Applicable Applications for anti-CGRP antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:500-1:1K
IHC-P: 4ug/ml
IHC-P: 4ug/ml
Conjugation
Unconjugated
Immunogen
15 amino acid synthetic peptide located withinthe human CGRP1(alpha-CGRP)sequence.
Preparation and Storage
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Western Blot (WB)
(CL7812AP (0.01ug/ml) staining of human skin (A) and MCF7 cell line (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(CL7812AP (0.01ug/ml) staining of human skin (A) and MCF7 cell line (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(CL7812AP (4ug/ml) staining of a human skin formalin-fixed, paraffin-embedded tissue section; seen at 20x (left) and 40x (right) magnification. Strong staining of collagen fibers observed in the extracellular space.)
Immunohistochemistry (IHC)
(CL7812AP (4ug/ml) staining of a human skin formalin-fixed, paraffin-embedded tissue section; seen at 20x (left) and 40x (right) magnification. Strong staining of collagen fibers observed in the extracellular space.)
Related Product Information for anti-CGRP antibody
Calcitonin gene-related peptide (CGRP) is a member of the calcitonin family of peptides, which in humans exists in two forms, alpha-CGRP and beta-CGRP. CGRP is produced in both peripheral and central neurons and is a potent peptide vasodilator and can function inthe transmission of nociception. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. Increased levels of CGRP have been reported in migraine and temporomandibular joint disorder patients as well as a variety of other diseases such as cardiac failure, hypertension, and sepsis. Several monoclonal antibody therapies targeting CGRP and it’s receptor have been developed for patients suffering from migraine attacks.
References
1. Amara SG, Jonas V, et al.(1982) Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products.Nature.298(5871): 240-4.
2. Rosenfeld MG, Mermod JJ, et al(1983) Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing.Nature.304(5922): 129-35.
3. Brain SD, Williams TJ, et al.(1985) Calcitonin gene-related peptide is a potent vasodilator.Nature.313(5997): 54-6.
4. Kitamura K. et al. (1992) Isolation and characterization of peptides which act on rat platelets, from a pheochromocytoma. Biochem. Biophys. Res. Commun. 185:134-141.
5. Bigal ME, Rapoport AM, et al.(2018). From LBR-101 to Fremanezumab for Migraine.CNS Drugs.32(11): 1025-1037.
2. Rosenfeld MG, Mermod JJ, et al(1983) Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing.Nature.304(5922): 129-35.
3. Brain SD, Williams TJ, et al.(1985) Calcitonin gene-related peptide is a potent vasodilator.Nature.313(5997): 54-6.
4. Kitamura K. et al. (1992) Isolation and characterization of peptides which act on rat platelets, from a pheochromocytoma. Biochem. Biophys. Res. Commun. 185:134-141.
5. Bigal ME, Rapoport AM, et al.(2018). From LBR-101 to Fremanezumab for Migraine.CNS Drugs.32(11): 1025-1037.
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Product Notes
The CGRP (Catalog #AAA524560) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human CGRP, Purified, (Polyclonal)(Goat IgG) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CGRP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:500-1:1K IHC-P: 4ug/ml. Researchers should empirically determine the suitability of the CGRP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CGRP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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