Western Blot (WB)
Rabbit cGKI Polyclonal Antibody | anti-cGKI antibody
cGKI Antibody
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
Western Blot (WB)
Western Blot (WB)
Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins.
NCBI and Uniprot Product Information
NCBI Description
Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]
Uniprot Description
PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described.
Protein type: Kinase, protein; Protein kinase, AGC; EC 2.7.11.12; Protein kinase, Ser/Thr (non-receptor); AGC group; PKG family
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: cytoplasm; cytosol; Golgi apparatus; plasma membrane
Molecular Function: ATP binding; calcium channel regulator activity; cGMP binding; cGMP-dependent protein kinase activity; protein binding; protein serine/threonine kinase activity
Biological Process: actin cytoskeleton organization and biogenesis; cGMP-mediated signaling; dendrite development; forebrain development; neuron migration; protein amino acid phosphorylation; regulation of GTPase activity; relaxation of vascular smooth muscle; signal transduction
Research Articles on cGKI
Similar Products
Product Notes
The cGKI prkg1 (Catalog #AAA540144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The cGKI Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's cGKI can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the cGKI prkg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cGKI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
