Rabbit anti-Human CFHR5 Polyclonal Antibody | anti-CFHR5 antibody

CFHR5 Antibody (Center)

Cat. Num. AAA9207573

• Gene Names: CFHR5; FHR5; CFHL5; FHR-5; CFHR5D
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CFHR5; Polyclonal Antibody; CFHR5 Antibody (Center); Complement factor H-related protein 5; FHR-5; CFHL5; FHR5; anti-CFHR5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB22505
• Specificity: This CFHR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-231 amino acids from the Central region of human CFHR5.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 203-231
• Sequence Length: 569

• Applicable Applications for anti-CFHR5 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry - Paraffin (IHC-P), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; IHC-P~~1:100; FC~~1:10~50
• Function: Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
• Cellular Location: Secreted.
• Tissue Location: Expressed by the liver and secreted in plasma.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(CFHR5 Antibody (Center) (MBS9207573) western blot analysis in CEM(lane 1),K562(lane 2) cell line lysates (15ug/lane). This demonstrates the CFHR5 antibody detected the CFHR5 protein (arrow).)

Immunohistochemistry (IHC)

(CFHR5 Antibody (Center) (MBS9207573) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CFHR5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CFHR5 Antibody (Center) (MBS9207573) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded H.liver tissue reacted with CFHR5 Antibody (Center) (MBS9207573).)

Related Product Information for anti-CFHR5 antibody

CFHR5 is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq].

Product Categories/Family for anti-CFHR5 antibody

Immunology

References

McRae, J.L., et al. Genetica 114(2):157-161(2002)
Murphy, B., et al. Am. J. Kidney Dis. 39(1):24-27(2002)
Narkio-Makela, M., et al. Clin. Immunol. 100(1):118-126(2001)
McRae, J.L., et al. J. Biol. Chem. 276(9):6747-6754(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 13540563
• NCBI GeneID: 81494
• NCBI Accession #: NP_110414.1
• NCBI GenBank Nucleotide #: NM_030787.3
• UniProt Accession #: Q9BXR6; Q2NKK2
• Molecular Weight: 64419
• NCBI Official Full Name: complement factor H-related protein 5
• NCBI Official Synonym Full Names: complement factor H-related 5
• NCBI Official Symbol: CFHR5
• NCBI Official Synonym Symbols: FHR5; CFHL5; FHR-5; CFHR5D
• NCBI Protein Information: complement factor H-related protein 5
• UniProt Protein Name: Complement factor H-related protein 5
• Protein Family: Complement factor H-related protein
• UniProt Gene Name: CFHR5
• UniProt Synonym Gene Names: CFHL5; FHR5; FHR-5
• UniProt Entry Name: FHR5_HUMAN

NCBI Description

This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq, Jan 2010]

Uniprot Description

CFHR5: Involved in complement regulation. Defects in CFHR5 have been found in patients with atypical hemolytic uremic syndrome and may contribute to the disease. Atypical hemolytic uremic syndrome is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: extracellular region

Biological Process: complement activation, alternative pathway

Disease: Cfhr5 Deficiency